Discover your roadmap to Multiplexing

While multiplexing is more cost-effective than running a series of single-analyte immunoassays, it introduces several technical challenges that make assay design more complicated. For example, individual ELISA assays could be optimised such that every component, from buffer to antibody pairs and more, could be custom-tailored for that specific analyte. However, multiplex assays don’t have this luxury due to incorporation of several analytes, various antibodies etc. Therefore, it is absolutely crucial to extensively design and test the various assay components to ensure analytes play well together and produce accurate, meaningful results. Inadequate assay design and testing could result in several aberrations such as displaying false positives, false negatives, or under-reporting values due to interference.

R&D Systems has over 30 years of experience developing and optimising immunoassays to control for these factors. We conduct the most rigorous quality control and as a result our assays outperform competitors’ assays in a number of dimensions, including natural sample linearity, lot-to-lot consistency, precision and reproducibility, sensitivity, and minimization of false positive signals.

To find out more about factors that influence assay performance and how R&D LUMINEX® outperforms competitors’ please download our summary PDF's below..

Click Here to Download the Luminex Multiplex Immunoassays Users Guide 

Click Here to get Superior Accuracy with R&D Systems Luminex Assays

What are Proteome Profiler™ Antibody Arrays?

Proteome Profiler Antibody Arrays are simple and economical multiplex assays for early-stage discovery research.

This semiquantitative antibody array allows for the measurement of more than 100 proteins in a single sample. It requires no specialized equipment and eliminates the need for multiple Western blot experiments. Antibody array kits contain buffers, detection antibodies, and membranes spotted in duplicate with high quality capture antibodies. The arrays utilize chemiluminescence for detection data that can be quantified in the same manner as traditional Western blots.

Select antibody arrays are also suitable for use with the LI COR® and FluorChem™ detection systems.

Antibody Array Principle

1. Capture antibodies are spotted in duplicate on the nitrocellulose membrane.
2. Spotted membrane arrays are incubated in a single lysate.
3. Target analytes are captured by the capture antibody.
4. Target analytes are detected using a biotinylated or horseradish peroxidase (HRP) conjugated detection antibody.
5. Biotinylated detection antibodies are reacted with streptavidin HRP.
6. Membranes are incubated in a chemiluminescence reagent that produces a light signal proportional to the amount of antibody target analyte complexes formed.
7. Data is visualized using standard western blotting methods such as film or digital cameras.

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