In Vitro Technologies

Introducing the innovative and novel CelVivo System for 3D cell culture to bring you unlimited tissue models.

The CelVivo system creates an environment which promotes the growth and maintenance of large 3D tissue mimetic structures, whether they are spheroids, organoids and other aggregates. The technology CelVivo has developed to grow cells so they retain or recover their in vivo physiological attributes.

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Win a ClinoStar 2 for a year!

To celebrate the recent launch, CelVivo is giving two unique labs worldwide the chance to work on their upcoming projects using the ClinoStar 2.

The system will include full access to the CelVivo Cloud Service and the Hypoxia add-on unit.

And the best part is, you will have all this as a free trial for one year!

Win a ClinoStar 2

The ClinoStar Technology

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Introducing the ClinoStar 2 – Connecting your research to in vivo

Accurately establishing and monitoring oxygen levels during the culture period is crucial for mimicking in vivo physiological conditions of tissues and organs.

Oxygen concentrations in the human body vary, ranging from 12% in the lungs to 5% in the brain, but can be as low as 0.1% in tumours. The all new ClinoStar hypoxia unit enables the manipulation of in vitro atmospheric composition to tailor it to the specific physiological needs of tissues/organs.

With the hypoxia module, you can:

• Regulate oxygen levels in ClinoStar 2
• Quick attainment of the media’s oxygen level set point
• Simplifies short-term handling procedures, such as microscopy observation and documentation

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CelVivo Cloud

With CelVivo Cloud you’ll control your ClinoStar remotely from any internet-enabled device – anywhere in the world!

The web-based software makes it easy to adjust the climate settings in your ClinoStar through control of temperature, CO₂, and O₂.

Clinostat for 3D cell culture

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The ClinoStar® system employs a clinostat and a rotating bioreactor to keep the cells suspended in culture media by counterbalancing the gravitational forces.

This approach has the advantage that the spheroids and organoids formed, are exposed to very low shear forces and have good gas and nutrient exchange. Low shear forces, combined with active diffusion, are key parameters for cells to develop into functional 3D constructs.

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The ClinoStar® is controlled by a tablet with pre-installed software, which allows you to adjust CO₂, temperature, and rotation speed of each bioreactor from 1 to 100 rpm in 0.1 rpm steps (clockwise or anticlockwise).

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ClinoReactor is a bioreactor with a fixed 10 mL culture chamber, that is supplied sterile. The humidification beads supply water for the culture chamber, which eliminate the need for water in the incubator. To prevent cell, protein and drug absorption, the bioreactor is made of low bind polypropylene and polystyrene. The optically clear properties of the polystyrene enables microscopy of the spheroids or organoids in a closed environment.

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How to work with ClinoStar®

Culturing spheroids and organoids is dependent on the cell model and sample being cultured. With the ClinoStar® system, we have created a system to generate spheroids and organoids in a reproducible fashion. The initial cell aggregation can be performed in several ways to accommodate various cell and sample types, but the downstream process remains the same.

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Applications of 3D cell culture

The ClinoStar® enables generation of uniform, reproducible and functional spheroids and organoids. These spheroids mimic the function, structure and architecture of in vivo cells. The ClinoStar® technology isn’t merely “3D cell culture”, it enables research using cell models with an unprecedented correlation between in vivo and in vitro conditions, and closes the gap between 2D cell culture and cell function.