Bio-techne: Resources and recipes for organoid and 3D culture
Organoid and 3D cell culture are emerging as pivotal systems for understanding human organ development, modelling disease, screening for drug efficacy or toxicity, and investigating personalised medicine. The reagents and protocols needed to culture these advanced multi-cellular in vitro tissues vary by organ and species, as well as whether they are being generated from tissue-specific adult stem cells or induced pluripotent stem cells (iPSCs).
This page serves as a reagent and technical resource from R&D Systems to help you build robust and consistent organoid cultures. Access protocols, exclusive webinars, organoid recipes, and discover new products relevant to your work in organoid research.
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R&D Systems products for physiologic cell culture recreate tissue microenvironments to elicit in vivo structure and function for cells in vitro. These tools for 2-D and 3-D cell culture are designed, developed, produced, qualified, and supported by R&D Systems’ Cultrex™ laboratory. This includes several unique Basement Membrane Extract (BME) matrices containing different compositions of extracellular matrix (ECM) proteins qualified for 3-D, stem cell, spheroid, and organoid cultures.
Cultrex™ Extracellular Matrix Free Sample Offer
Introducing Cultrex™ BME: The Dependable ECM
Carefully formulated to mimic the complex composition and physical properties of the native extracellular matrix, Cultrex Basement Membrane Extract (BME) matrices provide a physiologically relevant environment for your cells to grow and function in.
- Optimized Matrix Composition: Higher entactin concentration for enhanced tensile strength
- Minimize Contamination Risk: Low endotoxin profile (<8 EU/mL)
- Spend Less Time Testing: Stringently quality controlled to ensure high lot-to-lot consistency
- Easy Switching from Other Matrices: Our technical specialists are on hand to provide advice to enable a seamless transition from other cell culture matrices
Free Sample Offer - Terms and Conditions
- One sample per customer
- Limited and select samples available
- Quote discount code: MKT0526 to receive this offer
- Please kindly note that a $100 dry ice surcharge applies for delivery
Claim your Free Sample by filling in the form
Cultrex® Basement Membrane Extracts (BME) Product Features
Cultrex BMEs are a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. These extracts provide a natural extracellular matrix hydrogel that polymerizes at 37 °C to form a reconstituted basement membrane that is used to promote and maintain a differentiated phenotype of cell cultures such as epithelial cells, endothelial cells, glandular cells, neurons, and smooth muscle cells. BME can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion and in vivo tumorigenicity assays.
PRODUCT FEATURES
• Rapid - gels within 30 minutes at 37 °C.
• Reliable - maintains gelled form in culture medium for 14 days.
• Sterile - screened for bacteria, fungi, and mycoplasma.
• Specific - available with reduced growth factor content, with or without Phenol Red, and additional sterility testing.
Cultrex® BME Selection Guide
| Application | Name | Description | RGF Version Available? | Notes |
Organoids and Stem Cells
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NEW! Cultrex UltiMatrix RGF BME | Soluble form of basement membrane that provides high tensile strength, enhanced levels of entactin/nidogen, elevated protein concentration, and robust clarity and purity. | Yes | This matrix contains compositional enhancements and performance benefits for organoid and stem cell culture. |
Stem Cells
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Cultrex Stem Cell Qualified BME | Provides an effective feeder-free surface for the attachment and maintenance of stem cells in an undifferentiated state, enabling growth promotion or study of stem cell differentiation. | Yes | Attachment and maintenance of stem human embryonic and induced pluripotent stem cells. |
Stem Cells
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Cultrex ReadyBME, Stem Cell Qualified | Pre-diluted, pre-qualified ready-to-use basement membrane extract for feeder-free pluripotent stem cell maintenance and differentiation. | Yes | Improves experiment consistency and success by minimizing user handling and coating matrix dilution variability. |
3-D Tissue Spheroids
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Cultrex 3-D Culture Matrix BME* | BME used for modeling the morphological effects of early oncogenesis on 3-D microenvironments, wherein healthy, differentiating cells exhibit a structured, polarized morphology that is critical for tissue formation and function. Specifically produced for and qualified in 3-D culture studies. | Yes | 3-D cell cultures. |
Organoid
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Cultrex Organoid Qualified BME, Type 2 | A Reduced Growth Factor BME that has been developed and qualified specifically for robust organoid cultures. | Yes | Robust organoid cultures. |
Organoid
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Cultrex Organoid Qualified BME, Type R1 | A Reduced Growth Factor BME that has been developed and qualified specifically for difficult organoid cultures. | Yes | Difficult to culture organoids. |
Cancer/Tumorspheres
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Cultrex BME, Type 3* | BME Type 3 helps cells to adapt to in vivo transplantation. Mimicking the in vivo microenvironment, including low glucose and low pH, to improve take rate and growth of implanted cells for xenograft and tumorgraft models. | No | Xenograft/Tumorgrafts. |
General
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Cultrex BME* | Consists of laminin I, type IV collagen, entactin, and heparan sulfate proteoglycan. | Yes | General use including 2-D cultures, 3-D cultures, cell attachment and cell invasion. |
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Organoids Wall Poster
Organoids are emerging as powerful new platforms for exploring human organ development, modeling disease, screening for drug toxicity, and investigating personalized medicine. Request this poster to decorate your lab with some of the visually stunning organoid images that have been generated by cell and gene therapy scientists at Bio-Techne.