Agilent Seahorse XF Analysis uses label-free technology to detect discrete changes in cell bioenergetics in real-time, providing a window into the cellular behavior driving cell signalling, proliferation, activation, toxicity, and biosynthesis.
Agilent Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) at intervals of approximately 5-8 minutes. OCR is an indicator of mitochondrial respiration, and ECAR is largely the result of glycolysis.
Automated compound injection during the course of the experiment allows researchers to modulate cellular metabolism.
By combining these data, the Seahorse is uniquely able to metabolically profile cells and discover their hidden energetic potential.
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