Functional mitochondrial measurements are key to understanding cellular activation, proliferation, differentiation and dysfunction. The Seahorse XF Cell Mito Stress Test provides a complete mitochondrial profile, revealing critical information not evident in basal metabolism measurements alone. 

• Learn more about How XF Technology Works

Features

• Provides a robust test for analyzing the key parameters of mitochondrial function
• Measure: Basal respiration, ATP-linked respiration, H+ (Proton) Leak, Maximal Respiration, Spare Respiratory Capacity, and Non-mitochondrial respiration.
• Kit provides quality-controlled, pre-measured reagents and a standard method for conducting a Seahorse XF Cell Mito Stress Test
• Seahorse XF Report Generator automatically calculates the key parameters of your samples, simplifying data analysis
• Seahorse XF Cell Mito Stress Test Report Generator
• Let’s talk about Mitochondrial Respiration
• For Research Use Only. Not for use in diagnostic procedures.

Specifications

Literature

• Identifying Metabolic Switches Using an Agilent Seahorse XFe Analyzer in Hypoxia

Identifying Metabolic Phenotype Switches in Cancer Cells Using the Agilent Seahorse XF/XFe Analyzer in an Hypoxic Environment

• Measure Mitochondrial Respiration in Skeletal Muscle Fibers with a Seahorse Analyzer

Measuring Mitochondrial Respiration in Intact Skeletal Muscle Fibers with an Agilent Seahorse XF24/XFe24 Analyzer 

• Assessing mitochondrial dysfunction in primary cardiomyocytes

This Application Note describes a method for profiling mitochondrial function in cells responding to stress.