Adenosine A2a R Antibody (7F6-G5-A2) Summary
| Immunogen |
Full length recombinant human Adenosine A2a R. [UniProt# P29274] |
| Epitope |
Epitope mapped to SQPLPGER in the third intracellular loop (PMID 9822147). |
| Isotype |
IgG2a |
| Clonality |
Monoclonal |
| Host |
Mouse |
| Gene |
ADORA2A |
| Purity |
Protein G purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.Learn about the Innovator's Reward
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Applications/Dilutions
| Dilutions |
- Western Blot 1:1000
- Flow Cytometry 1-5 ug/mL
- Immunocytochemistry/Immunofluorescence 1:50-1:100
- Immunohistochemistry 1 -5 ug/ml
- Immunohistochemistry-Frozen
- Immunohistochemistry-Paraffin 1 -5 ug/ml
- CyTOF-ready
- Flow (Intracellular)
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| Application Notes |
ICC usage is reported in scientific literature (PMID: 25836920). Use in IHC-frozen secions reported in scientific literature (PMID 9822147). |
| Theoretical MW |
43 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Publications |
| Read Publications using NBP1-39474 in the following applications: |
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Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
PBS |
| Preservative |
0.05% Sodium Azide |
| Concentration |
1.0 mg/ml |
| Purity |
Protein G purified |
Alternate Names for Adenosine A2a R Antibody (7F6-G5-A2)
- A2aR
- adenosine A2 receptor
- Adenosine A2a R
- adenosine A2a receptor
- Adenosine A2aR
- adenosine receptor A2a
- adenosine receptor subtype A2a
- ADORA 2
- ADORA2
- ADORA2A
- hA2aR
- RDC8
Background
This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G-protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for Adenosine A2a R Antibody (NBP1-39474)(9)
We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.We have publications tested in 6 applications: FLOW, Flow, ICC, ICC/IF, IHC-Fr, WB.
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Filter By Application
FLOW (1)
Flow (1)
ICC (1)
ICC/IF (2)
IHC-Fr (1)
WB (5)
All Applications
Filter By Species
Human (4)
Mouse (2)
Rat (2)
Porcine (1)
All Species
Showing Publications 1 -
9 of 9.
| Publications using NBP1-39474 |
Applications |
Species |
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| Mediavilla-Varela M, Castro J, Chiappori A et al. A Novel Antagonist of the Immune Checkpoint Protein Adenosine A2a Receptor Restores Tumor-Infiltrating Lymphocyte Activity in the Context of the Tumor Microenvironment. Neoplasia. 2017 Jun 02 [PMID: 28582704] (Flow, Human) |
Flow |
Human |
| Nguyen KD. Inflammatory Cytokines Regulate Adenosine A2A Receptor Expression, Function, and Desensitization. Book from NEW YORK UNIVERSITY. 2007 |
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| Clancy-Thompson E, Perekslis TJ, Croteau W et al. Melanoma Induces, and Adenosine Suppresses, CXCR3-Cognate Chemokine Production and T-cell Infiltration of Lungs Bearing Metastatic-like Disease. Cancer Immunol Res. 2015 Aug 1 [PMID: 26048575] (FLOW, Mouse, Human)Details:Adenosine A2a R antibody (clone 7F6-G5-A2) was used for FLOW analysis of adenosine receptor A2A (ADORA2A) in murine B16 melanoma and human VMM12 melanoma cells (Figure 7). |
FLOW |
Mouse, Human |
| Del Ry S, Moscato S, Bianchi F et al. Altered expression of connexin 43 and related molecular partners in a pig model of left ventricular dysfunction with and without dipyrydamole therapy Pharmacol. Res. 2015 Mar 30 [PMID: 25836920] (ICC/IF, Rat) |
ICC/IF |
Rat |
| Ribeiro AS, Fernandes VS, Orensanz LM, Martinez MP, Recio P, Martinez-Saenz A, Climent B, Arteaga JL, Garcia-Sacristan A, Prieto D, Hernandez M. Mechanisms involved in the adenosine-induced vasorelaxation to the pig prostatic small arteries. Purinergic Signal;7(4):413-25. 2011 Dec. [PMID: 21567127] (WB, Porcine) |
WB |
Porcine |
| Haschemi A, Wagner O, Marculescu R et al. Cross-regulation of carbon monoxide and the adenosine A2a receptor in macrophages. J Immunol. 2007 May 1 [PMID: 17442976] (WB, ICC/IF, Mouse) |
WB, ICC/IF |
Mouse |
| Nguyen DK, Montesinos MC, Williams AJ et al. Th1 cytokines regulate adenosine receptors and their downstream signaling elements in human microvascular endothelial cells. J Immunol. 2003 Oct 15 [PMID: 14530318] (WB, Human)Details:Adenosine A2a R antibody (clone 7F6-G5-A2) was used for Western blot analysis of adenosine A2A receptors in Human Microvascular Endothelial Cells (HMVECs) which were treated overnight with IL-1, TNF-alpha, or IFN-gamma. |
WB |
Human |
| Khoa ND, Montesinos MC, Reiss AB et al. Inflammatory cytokines regulate function and expression of adenosine A(2A) receptors in human monocytic THP-1 cells. J Immunol. 2001 Oct 1 [PMID: 11564822] (WB, Human)Details:Adenosine A2a R antibody (clone 7F6-G5-A2) was used for Western blot analysis of crude membrane protein lysates from THP-1 cells which were treated or not with IL1, TNF-alpha or IFN-gamma. |
WB |
Human |
| Rosin DL, Robeva A, Woodard RL et al. Immunohistochemical localization of adenosine A2A receptors in the rat central nervous system. J Comp Neurol. 1998 Nov 16 [PMID: 9822147] (WB, ICC, IHC-Fr, Rat) |
WB, ICC, IHC-Fr |
Rat |
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Western Blot
- Immunohistochemistry-Frozen
- Flow Cytometry
- Immunocytochemistry/Immunofluorescence
- Immunohistochemistry-Paraffin
- View all Protocols, Troubleshooting, Illustrated assays and Webinars.
Video Protocols
WB Video Protocol
ICC/IF Video Protocol
FAQs for Adenosine A2a R Antibody (NBP1-39474). (Showing 1 - 1 of 1 FAQs).
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What is the concentration of Adenosine A2a R Antibody (7F6-G5-A2) [Alexa Fluor® 647]
- We routinely use 0.1 mg of base antibodies (i.e. unconjugated antibody like NBP1-39474) in our conjugation reactions with the dyes/enzymes of interest. And the recovery rates mostly fall between 85 to 100% and hence the concentrations are about 0.80 - 1.0 mg/ml, in 0.1 ml volume.
Secondary Antibodies
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Isotype Controls
- Mouse Isotype Controls
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- View all of our Isotype Control Products
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Other Available Formats
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Alexa Fluor 405 Labeled
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NBP1-39474AF405
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Alexa Fluor 488 Labeled
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NBP1-39474AF488
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Alexa Fluor 647 Labeled
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NBP1-39474AF647
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Alexa Fluor 700 Labeled
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NBP1-39474AF700
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Allophycocyanin Labeled
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NBP1-39474APC
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DyLight 350 Labeled
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NBP1-39474UV
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DyLight 405 Labeled
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NBP1-39474V
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DyLight 488 Labeled
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NBP1-39474G
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DyLight 680 Labeled
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NBP1-39474FR
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DyLight 755 Labeled
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NBP1-39474IR
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PE Labeled
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NBP1-39474PE
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PerCP Labeled
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NBP1-39474PCP
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Learn about Custom Antibody Labeling
Additional Array Products
Array NBP1-39474
- Adenosine A2a R Antibodies
- Adenosine A2a R Antibody Pair
- Adenosine A2a R ELISA Kit
- Adenosine A2a R Lysates
- Adenosine A2a R Proteins
- Adenosine A2a R RNAi
Bioinformatics Tool for Adenosine A2a R Antibody (NBP1-39474)
Discover related pathways, diseases and genes to Adenosine A2a R Antibody (NBP1-39474). Need help?
Read the Bioinformatics Tool Guide for instructions on using this tool.
Diseases for Adenosine A2a R Antibody (NBP1-39474)
Discover more about diseases related to Adenosine A2a R Antibody (NBP1-39474).
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Pathways for Adenosine A2a R Antibody (NBP1-39474)
View related products by pathway.
- Pathogenesis
- Transport
- Dna Methylation
- Methylation
- Cell Activation
- Aging
- Neurogenesis
- Response To Caffeine
- Reflex
- Reverse Transcription
- System Development
- Paracrine Signaling
- Cognition
- Central Nervous System Development
- Lymphocyte Proliferation
- Chromatin Remodeling
- Prepulse Inhibition
- Menopause
- Adenosine Transport
- Â
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PTMs for Adenosine A2a R Antibody (NBP1-39474)
Learn more about PTMs related to Adenosine A2a R Antibody (NBP1-39474).
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Research Areas for Adenosine A2a R Antibody (NBP1-39474)
Find related products by research area.
- Apoptosis
- GPCR
- Immunology
- Innate Immunity
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Blogs on Adenosine A2a R.
Check out the latest blog posts on Adenosine A2a R.
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Adenosine Inhibits T cell Tumor Infiltration: KCa3.1, a New Anticancer Target By Yoskaly Lazo-Fernandez, PhD Role of Adenosine in the Tumor Microenvironment a Target for Cancer TherapyThe tumor microenvironment (TME) tends to be concentrated in the purine nucleoside adenosine, a direct resu...  Read full blog post. |
