A20; B Cell Lymphoma; Mouse (Mus musculus) Item ID: ATCTIB208
| Permits and Restrictions |
View Permits |
|---|---|
| Organism | Mus musculus, mouse |
| Cell Type | B lymphocyte |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | reticulum cell sarcoma |
| Strain | BALB/cAnN |
| Applications | This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | diploid; modal number = 37; range = 33 to 38 RefKim KJ, et al. Establishment and characterization of BALB/c lymphoma lines with B cell properties. J. Immunol. 122: 549-554, 1979. PubMed: 310843 |
|---|---|
| Derivation | The A20 cell line is a BALB/c B cell lymphoma line derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse. |
| Antigen Expression | Antigen expression: I-A+ RefKim KJ, et al. Establishment and characterization of BALB/c lymphoma lines with B cell properties. J. Immunol. 122: 549-554, 1979. PubMed: 310843 |
| Receptor Expression | Fc |
| Genes Expressed | immunoglobulin (surface, sIg+),I-A+ |
| Cellular Products | immunoglobulin (surface, sIg+) |
| Tumorigenic | Yes |
| Effects | Yes |
| Comments | The cells express little surface immunoglobulin when grown in Click's medium; however, they express large amounts when grown in RPMI 1640 medium. The cells can present both alloantigens and protein antigens. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
|
|---|---|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation | culture medium 95%; DMSO, 5% |
| Culture Conditions | Temperature: 37°C |
| Isotype | IgG |
|---|---|
| Population Doubling Time | 18 hrs |
| Name of Depositor | KJ Kim |
|---|---|
| References |
Kim KJ, et al. Establishment and characterization of BALB/c lymphoma lines with B cell properties. J. Immunol. 122: 549-554, 1979. PubMed: 310843 Glimcher LH, et al. Ia antigen-bearing B cell tumor lines can present protein antigen and alloantigen in a major histocompatibility complex-restricted fashion to antigen-reactive T cells. J. Exp. Med. 155: 445-459, 1982. PubMed: 6460073 Mallick-Wood CA, et al. Disruption of epithelial gamma delta T cell repertoires by mutation of the Syk tyrosine kinase. Proc. Natl. Acad. Sci. USA 93: 9704-9709, 1996. PubMed: 8790395 Hartley D, Corvera S. Formation of c-Cb1-phosphatidylinositol 3-kinase complexes on lymphocyte membranes by a p56lck-independent mechanism. J. Biol. Chem. 271: 21939-21943, 1996. PubMed: 8702998 Kim KJ, et al. Establishment and characterization of BALB/c lymphoma lines with B cell properties. J. Immunol. 122: 549-554, 1979. PubMed: 310843 |
| Cross References |
Nucleotide (GenBank) : M58558 Murine sm D small nuclear ribonucleoprotein sequence. Nucleotide (GenBank) : NM_009226 Mus musculus small nuclear ribonucleoprotein D1 (Snrpd1), mRNA. |