THP-1; Acute Monocytic Leukemia; Human (Homo sapiens) Item ID: ATCTIB202
| Organism | Homo sapiens, human |
|---|---|
| Tissue | peripheral blood |
| Cell Type | monocyte |
| Product Format | frozen |
| Morphology | monocyte |
| Culture Properties | suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | acute monocytic leukemia |
| Age | 1 year infant |
| Gender | male |
| Applications | These cells are suitable as a transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images | |
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| Antigen Expression | HLA A2, A9, B5, DRw1, DRw2 ref |
| Receptor Expression | complement (C3), expressed ref Fc, expressed |
| Genes Expressed | lysozyme,HLA A2, A9, B5, DRw1, DRw2 |
| Cellular Products | lysozyme |
| Comments | The cells are phagocytic (for both latex beads and sensitized erythrocytes) and lack surface and cytoplasmic immunoglobulin. Monocytic differentiation can be induced with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2-4 x 105 viable cells/mL. Subculture when cell concentration reaches 8x105 cells/mL.Do not allow the cell concentration to exceed 1 x 106 cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product. Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 11,13 D13S317: 13 D16S539: 11,12 D5S818: 11,12 D7S820: 10 THO1: 8,9.3 TPOX: 8,11 vWA: 16 |
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| Name of Depositor | S Tsuchiya |
|---|---|
| References |
Tsuchiya S, et al. Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res. 42: 1530-1536, 1982. PubMed: 6949641 Skubitz KM, et al. Human granulocyte surface molecules identified by murine monoclonal antibodies. J. Immunol. 131: 1882-1888, 1983. PubMed: 6619543 Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019 Huang S, et al. Adenovirus interaction with distinct integrins mediates separate events in cell entry and gene delivery to hematopoietic cells. J. Virol. 70: 4502-4508, 1996. PubMed: 8676475 Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679 Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116 Hsu HY, et al. Inhibition of macrophage scavenger receptor activity by tumor necrosis factor-alpha is transcriptionally and post-transcriptionally regulated. J. Biol. Chem. 271: 7767-7773, 1996. PubMed: 8631819 Lucas M, Mazzone T. Cell surface proteoglycans modulate net synthesis and secretion of macrophage apolipoprotein E. J. Biol. Chem. 271: 13454-13460, 1996. PubMed: 8662812 Sando GN, et al. Induction of ceramide glucosyltransferase activity in cultured human keratinocytes. J. Biol. Chem. 271: 22044-22051, 1996. PubMed: 8703011 Ollivier V, et al. Elevated cyclic AMP inhibits NF-kappaB-mediated transcription in human monocytic cells and endothelial cells. J. Biol. Chem. 271: 20828-20835, 1996. PubMed: 8702838 |
| Cross References |
Nucleotide (GenBank) : X70326 H.sapiens MacMarcks mRNA. |