In Vitro Technologies

Permits and Restrictions	View Permits
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications	Penicillin-Streptomycin-Amphotericin B Solution is used to reduce the chance of microbial contamination while propagating primary cells or continuous cell lines in culture.
Product Format	frozen 1.0 mL
Storage Conditions	Store the Penicillin -Streptomycin -Amphotericin B Solution at -20°C or colder. When stored as directed, the product is stable until the expiration date on the label. It should be thawed only one time.

Comments	This antibiotic solution is formulated as follows: Penicillin 10,000 Units/mL, Streptomycin 10 mg/mL, Amphotericin B 25 µg/mL.

Complete Growth Medium	Take the vial from storage and thaw. Mix well or vortex briefly to make sure the solution is homogenous. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the appropriate volume of the antibiotic/antimycotic solution to complete media. Dilute 1:1000 when using to supplement complete growth media; i.e., 0.5 mL of Penicillin-Streptomycin-Amphotericin B Solution per 500 mL of complete cell-specific media. Dilute 1:100 when using during cell isolation procedures; i.e., 0.5 mL of Penicillin-Streptomycin-Amphotericin B Solution per 50 mL of complete cell-specific growth media. Tightly cap the bottle (or other flask) of supplemented growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the flask. Fully supplemented media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, fully supplemented media is stable for 30 days.

Complete Growth Medium

Take the vial from storage and thaw. Mix well or vortex briefly to make sure the solution is homogenous.
Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the appropriate volume of the antibiotic/antimycotic solution to complete media.
Dilute 1:1000 when using to supplement complete growth media; i.e., 0.5 mL of Penicillin-Streptomycin-Amphotericin B Solution per 500 mL of complete cell-specific media.
Dilute 1:100 when using during cell isolation procedures; i.e., 0.5 mL of Penicillin-Streptomycin-Amphotericin B Solution per 50 mL of complete cell-specific growth media.
Tightly cap the bottle (or other flask) of supplemented growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the flask.
Fully supplemented media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, fully supplemented media is stable for 30 days.

Volume	1.0 mL
Sterility Tests	Negative for bacteria, fungi and yeast.
Functional Tests	Each lot is assessed for acceptable growth of human primary cells compared to control. A Certificate of Analysis (COA) is available upon request for each lot of Penicillin-Streptomycin-Amphotericin B Solution.
C of A	Certificate of Analysis

Permits	These permits may be required for shipping this product to Australia: DAFF Import Permit formerly known as AQIS Import Permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis SDS

Permits

These permits may be required for shipping this product to Australia:

DAFF Import Permit formerly known as AQIS Import Permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment.

Basic Documentation

Product Sheet Certificate of Analysis SDS

Penicillin-Streptomycin-Amphotericin B Solution Item ID: ATCPCS999002