Permits and Restrictions |
View Permits |
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Organism | Homo sapiens, human |
Tissue | Foreskin |
Cell Type | Fibroblast |
Morphology | Spindle-shaped; cells are bipolar and refractile. |
Growth Properties | Adherent |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease | Normal |
Age | Neonatal |
Gender | Male |
Ethnicity | Batch-specific |
Applications | Response to pathogens, skin aging, wound healing, gene delivery, skin diseases (e.g., scleroderma) |
Product Format | frozen 1 mL |
Storage Conditions | -130°C or below |
Comments | Serum-free medium supports excellent growth curves and normal morphology, as well as serum-free (not animal-free) experimental conditions. The presence of 2% fetal bovine serum in the Fibroblast Growth Kit-Low serum (ATCC PCS-201-041) supports more prolific growth. |
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Complete Growth Medium |
Table 1. If using the Fibroblast Growth Kit–Serum-Free (ATCC® PCS-201-040), add the indicated volume for each component in the order shown.
Table 2. If using the Fibroblast Growth Kit–Low Serum (ATCC® PCS-201-041), add the indicated volume for each of the following components:
Antimicrobials and phenol red are not required for proliferation, but may be added if desired. The recommended volume of each optional component to be added to the complete growth media is summarized in Table 3.
Table 3. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)
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Subculturing |
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Volume | 1 mL |
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Sterility Tests | Bacteria: Negative Yeast: Negative Mycoplasma: Negative |
Viral Testing | Hepatitis B: Negative Hepatitis C: Negative HIV: Negative |
Viability | ≥ 50% when thawed from cryopreservation |
Population Doubling Capacity | ≥ 10 in complete growth medium |
C of A | Certificate of Analysis |
Permits |
These permits may be required for shipping this product to Australia:
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Basic Documentation | Product Sheet Certificate of Analysis SDS |
References |
Ng C, et al. Enhanced ex vivo expansion of adult mesenchymal stem cells by fetal mesenchymal stem cell ECM. Biomaterials 35(13): 4046-4057, 2014. PubMed: 24560460 Merrilees M, et al. Use of versican variant V3 and versican antisense expression to engineer cultured human skin containing increased content of insoluble elastin. J. Tissue Eng. Regen. Med. 2014. PubMed: 24945362 Fontaine K, Camarda R, Lagunoff M. Vaccinia virus requires glutamine but not glucose for efficient replication. J. Virol. 88(8): 4366-4374, 2014. PubMed: 24501408 Kulkarni A, Diehl-Jones W, Ghanbar S, Liu S. Layer-by-layer assembly of epidermal growth factors on polyurethane films for wound closure. J. Biomater. Appl. 29(2): 278-290, 2014. PubMed: 24525716 Toledo‐Piza A, Nakano E, Rici R, Maria D. Proliferation of fibroblasts and endothelial cells is enhanced by treatment with Phyllocaulis boraceiensis mucus. Cell Prolif. 46(1): 97-108, 2014. PubMed: 23278963 Farran A, et al. Design and characterization of a dynamic vibrational culture system. J Tissue Eng. Regen. Med. 7(3): 213-225, 2013. PubMed: 22095782 Lee J, et al. Proteomic profiling reveals upregulated protein expression of hsp70 in keloids. Biomed. Res. Int., 2013. PubMed: 24260741 Sow W, Lui Y, Ng K. Electrospun human keratin matrices as templates for tissue regeneration. Nanomedicine 8(4): 531-541, 2013. PubMed: 23560405 Du Z, Yang C, Rothschild M, Ross J. Novel microRNA families expanded in the human genome. BMC Genomics 14: 98, 2013. PubMed: 23402294 Erazo-Oliveras A, et al. Protein delivery into live cells by incubation with an endosomolytic agent. Nat. Methods 11(8): 861-867, 2014. PubMed: 24930129 |