MC/9; Liver Mast Cells; Mouse (Mus musculus) Item ID: ATCCRL8306
| Permits and Restrictions |
View Permits |
|---|---|
| Organism | Mus musculus, mouse |
| Tissue | liver |
| Cell Type | mast cell |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | 13 days |
| Strain | (B6 X A/J)F1 |
| Storage Conditions | liquid nitrogen vapor phase |
| Disclosure | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation | This is a cloned mast cell line derived from the fetal liver of a (B6 X A/J)F1 mouse. |
|---|---|
| Receptor Expression | FcERI (Fc of IgE) |
| Genes Expressed | histamine; leukotrienes |
| Cellular Products | histamine; leukotrienes |
| Comments |
The cells can be sensitized to specific antigens by incubating them with IgE having the desired antigenic specificity.
The cells so coated will release soluble mediators (such as histamine) in response to exposure to the antigen (allergen).
|
| Complete Growth Medium | Dulbecco's Modified Eagle's Medium with 4 mM L-glutamine adjusted to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonate and supplemented with 2 mM L-glutamine, 0.05 mM 2-mercaptoethanol, 10% Rat T-STIM (Becton Dickenson Catalog No. 354115) and 10% fetal bovine serum. |
|---|---|
| Subculturing | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 5 X 105 viable cells/ml. Maintain cell density between 2 X 105 and 2 X 106 viable cells/mL. Do not allow the cell density to exceed 2 X 106 cells/mL. Medium Renewal: Every 2 to 3 days |
| Cryopreservation | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions | Temperature: 37°C |
| Name of Depositor | Dana Farber Cancer Inst. |
|---|---|
| U.S. Patent Number | 4,559,310 |
| Disclosure | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| References |
Cantor HI, Nabel G. Assay methods and systems utilizing mast cell clones. US Patent 4,559,310 dated Dec 17 1985 Nabel G, et al. Use of cloned populations of mouse lymphocytes to analyze cellular differentiation. Cell 23: 19-28, 1981. PubMed: 6971154 Galli SJ, et al. Mast cell clones: a model for the analysis of cellular maturation. J. Cell Biol. 95: 435-444, 1982. PubMed: 6216259 Lewin I, et al. Aggregation of the FcepisilonRI in mast cells induces the synthesis of fos-interacting protein and increases its DNA binding activity: the dependence on protein kinase c-beta. J. Biol. Chem. 271: 1514-1519, 1996. PubMed: 8576146 Ishizuka T, et al. Aggregation of the FceRI on mast cells stimulates c-Jun amino-terminal kinase activity. J. Biol. Chem. 271: 12762-12766, 1996. PubMed: 8662803 |