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STC-1; Intestinal Neuroendocrine Cells; Mouse (Mus musculus) Item ID: ATCCRL3254

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Organism Mus musculus, mouse
Tissue intestine
Cell Type intestinal neuroendocrine tumor cells
Product Format frozen 1.0 mL
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease invasive small intestinal neuroendocrine carcinoma
Age 10 to 13 weeks
Strain C57B1/6J
Applications

This cell line may be a useful model for human neuroendocrine neoplasms of the gut, and useful tools for studying hormone secretin.
Storage Conditions liquid nitrogen vapor phase
Derivation The STC-1 cell line was derived from the intestinal tumors of RIP1Tag2/Rip2pyST1 double transgenic mice.
Cellular Products secretin
Comments The STC-1 cell line was derived from the intestinal tumors of double transgenic mice. Transgenic mice harboring a hybrid gene linking the rat insulin promoter (RIP) to polyoma small T (PyST) antigen were mated with transgenic mice harboring rat insulin promoter (RIP) linked to SV40 early region (Tag) creating off-spring harboring both transgenes (double transgenics). These mice were found to have frequent intestinal tumors in addition to pancreatic Beta-cell tumors. Gene expression studies suggested that the intestinal and pancreatic tumors arose as separate entities. The STC-1cell line produces the hormone secretin. This cell line may be a useful model for human endocrine neoplasms of the gut.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Cells must be subcultured when they reach ~70% confluence, or else they start to come off the flask into suspension. Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 
    1. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC 30-2200) or 0.05% Trypsin – 0.02% EDTA (ATCC PCS-999-003) solution to remove all traces of serum which contains trypsin inhibitor. 
    2. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 
    3. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 
    4. Transfer cell suspension to a centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. 
    5. Discard supernatant. Resuspend the cell pellet in fresh growth medium. 
    6. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C.
    Subcultivation Ratio: 1:3 to 1:5 is recommended.Medium Renewal: Every 2 to 3 days
    Cryopreservation Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSOStorage temperature: liquid nitrogen vapor phase
    Culture Conditions Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Cells per Vial 1.0 x 10^6
    Volume 1.0 mL
    Name of Depositor Douglas Hanahan, PhD Cold Spring Harbor Laboratory
    Year of Origin June 1990
    References

    Rindi G, et al. Development of Neuroendocrine Tumors in the Gastrointestinal Tract of Transgenic Mice. Am J Pathol 136(6): 1349-1363, 1990. PubMed: 2162628

    Grant SGN, et al. Early Invasiveness Characterizes Metastatic Carcinoid Tumors in Transgenic Mice. Cancer Res 51: 4917-4923, 1991. PubMed: 1654206

    Permits

    These permits may be required for shipping this product to Australia:

    • DAFF Import Permit formerly known as AQIS Import Permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment.
    Basic Documentation Product Sheet Certificate of Analysis SDS
    References

    Rindi G, et al. Development of Neuroendocrine Tumors in the Gastrointestinal Tract of Transgenic Mice. Am J Pathol 136(6): 1349-1363, 1990. PubMed: 2162628

    Grant SGN, et al. Early Invasiveness Characterizes Metastatic Carcinoid Tumors in Transgenic Mice. Cancer Res 51: 4917-4923, 1991. PubMed: 1654206

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