In Vitro Technologies

Permits and Restrictions	View Permits
Organism	Mus musculus, mouse
Cell Type	methylcholanthrene induced
Product Format	frozen
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	fibrosarcoma
Strain	C57BL/6J
Applications	The cell line has been used extensively since 1976 as an H-2b cell line for cell-mediated cytotoxicity assays; the cells express both H-2Kb and H-2Db. This cell line is the laboratory standard in many laboratories for mouse viral immunity studies. The MC57G is a fibrosarcoma cell line established from a tumor arising in a C57BL/6 mouse after treatment with methylcholanthrene.
Storage Conditions	liquid nitrogen vapor phase

Derivation	The MC57G is a fibrosarcoma cell line established from a tumor arising in a C57BL/6 mouse after treatment with methylcholanthrene. The cell line has been used extensively since 1976 as an H-2b cell line for cell-mediated cytotoxicity assays; the cells express both H-2Kb and H-2Db. This cell line is the laboratory standard in many laboratories for mouse viral immunity studies. MC57G is infectible by viruses.
Comments	The MC57G is a fibrosarcoma cell line established from a tumor arising in a C57BL/6 mouse after treatment with methylcholanthrene. The cell line has been used extensively since 1976 as an H-2b cell line for cell-mediated cytotoxicity assays; the cells express both H-2Kb and H-2Db. This cell line is the laboratory standard in many laboratories for mouse viral immunity studies. MC57G is infectible by viruses.

Derivation

The MC57G is a fibrosarcoma cell line established from a tumor arising in a C57BL/6 mouse after treatment with methylcholanthrene. The cell line has been used extensively since 1976 as an H-2b cell line for cell-mediated cytotoxicity assays; the cells express both H-2Kb and H-2Db. This cell line is the laboratory standard in many laboratories for mouse viral immunity studies. MC57G is infectible by viruses.

Comments

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation	Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C

Name of Depositor	B Knowles
Year of Origin	1976
References	Aden DP, Knowles BB. Cell surface antigens coded by the human chromosome 7. Immunogenetics 3: 209-221, 1976. Trinchieri G, et al. Cell-mediated cytotoxicity to SV40-specific tumour-associated antigens. Nature 261: 312-314, 1976. PubMed: 179019 Doherty PC, et al. H-2 gene expression in required for T cell-mediated lysis of virus-infected target cells. Nature 266: 361-362, 1977. PubMed: 300845 Doherty PC, et al. Cytotoxic T-cell responses in mice infected with influenza and vaccinia viruses vary in magnitude with H-2 genotype. J. Exp. Med. 148: 534-543, 1978. PubMed: 100569 Wiktor TJ, et al. In vitro evidence of cell-mediated immunity after exposure of mice to both live and inactivated rabies virus. Proc. Natl. Acad. Sci. USA 74: 334-338, 1977. PubMed: 299948 This cell line was established from a tumor which developed after treatment with methylcholanthrene.

Name of Depositor

B Knowles

Year of Origin

1976

References

Aden DP, Knowles BB. Cell surface antigens coded by the human chromosome 7. Immunogenetics 3: 209-221, 1976.

Trinchieri G, et al. Cell-mediated cytotoxicity to SV40-specific tumour-associated antigens. Nature 261: 312-314, 1976. PubMed: 179019

Doherty PC, et al. H-2 gene expression in required for T cell-mediated lysis of virus-infected target cells. Nature 266: 361-362, 1977. PubMed: 300845

Doherty PC, et al. Cytotoxic T-cell responses in mice infected with influenza and vaccinia viruses vary in magnitude with H-2 genotype. J. Exp. Med. 148: 534-543, 1978. PubMed: 100569

Wiktor TJ, et al. In vitro evidence of cell-mediated immunity after exposure of mice to both live and inactivated rabies virus. Proc. Natl. Acad. Sci. USA 74: 334-338, 1977. PubMed: 299948

This cell line was established from a tumor which developed after treatment with methylcholanthrene.

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MC57G; Fibrosarcoma; Mouse (Mus musculus) Item ID: ATCCRL2295

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