CCD-34LU (LUNG, DIPLOID, HUMAN) Item ID: ATCCRL1491
| Permits and Restrictions |
View Permits |
|---|---|
| Tissue | lung |
| Cell Type | fibroblast |
| Product Format | frozen |
| Morphology | fibroblast |
| Culture Properties | adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | normal |
| Age | 2.5 weeks |
| Gender | female |
| Ethnicity | Black |
| Applications | The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility). A normal skin line derived from the same patient is available as ATCC CRL-1497 |
| Derivation | The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility). A normal skin line derived from the same patient is available as ATCC CRL-1497 |
|---|---|
| Clinical Data | The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility). A normal skin line derived from the same patient is available as ATCC CRL-1497 female Black 2.5 weeks |
| Comments | The line was derived from normal lung tissue from a child who died of erythroblastosis (Rh incompatibility). A normal skin line derived from the same patient is available as ATCC CRL-1497 |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. |
|---|---|
| Subculturing | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 7 days. |
| Culture Conditions | Temperature: 37.0°C |
| STR Profile | Amelogenin: X CSF1PO: 11,13 D13S317: 9,13 D16S539: 8,13 D5S818: 12 D7S820: 10 THO1: 7,9 TPOX: 11 vWA: 15,16 |
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| Name of Depositor | GP Maxwell |
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