Neuronal Progenitor Cell; Origin ATCC-BXS0117; Normal,Human (Homo sapiens) Item ID: ATCACS5003
| Permits and Restrictions |
View Permits View Restrictions |
|---|---|
| Organism | Homo sapiens, human |
| Tissue | bone marrow CD34+ cells |
| Cell Type | neural progenitor cells |
| Product Format | frozen 1.0 mL per vial |
| Morphology | short spindle shape |
| Culture Properties | adherent |
| Biosafety Level |
2 [Cells contain Sendai viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | normal |
| Age | 27 |
| Gender | female |
| Ethnicity | Chinese |
| Applications | Neuronal differentiation and drug screen |
| Storage Conditions | Liquid nitrogen vapor phase (-130°C or colder) |
| Images | |
|---|---|
| Derivation | Normal NPCs (ATCC® ACS-5003™) are derived from ATCC-BXS0117 Human [Asian Female] Induced Pluripotent Stem Cells (ATCC®ACS-1031™) |
| Antigen Expression | Nestin, Pax-6 |
| Comments | Astrocyte, oligodendrocyte, and neuron differentiation; drug screening |
| Complete Growth Medium |
Complete growth media for Neural Progenitor Cells (NPCs) includes DMEM: F12 (ATCC®30-2006) supplemented with the Growth Kit for Neural Progenitor Cell Expansion (ATCC®ACS-3003). To make complete NPC medium add the following components of the kit to 464 mL DMEM: F12: 5 mL L-Alanyl-L-Glutamine 5 mL Non-Essential Amino Acids 10 mL NPC Growth Kit Component A 5 mL NPC Growth Kit Component B 1 mL NPC Growth Kit Component C 10 mL NPC Growth Kit Component D |
|---|---|
| Seeding Density | Post-thaw: 80,000 viable cells/cm2 on CellMatrix-coated dishes/plates.Subculture: 40,000 viable cells/cm2 on CellMatrix-coated dishes/plates. |
| Subculturing |
Post thaw day 1, perform a 100% medium change and remove all cells that did not attach. Perform a 100% medium change every other day thereafter. Passage the cells cells with diluted Accutase (50% Accutase and 50% DPBS) when they reach ~95% confluence and reseed the NPCs at 40,000 viable cells/cm2 on CellMatrix-coated dishes/plates. |
| Cryopreservation | Stem Cell Freezing Media (ATCC® ACS-3020™) |
| Culture Conditions |
Coat plates with CellMatrix (ATCC®ACS-3035) and culture the NPCs with NPC Growth Medium (ATCC® ACS-3003). |
| Cells per Vial | >1,000,000 cells per vial |
|---|---|
| Volume | 1.0 mL per vial |
| STR Profile | Amelogenin: X CSF1PO: 10,12 D13S17: 9,12 D16S539: 11 D5S818: 12 D7S820: 9,13 TH01: 7,9 TPOX: 8,11 vWA: 19 |
| Sterility Tests |
No bacterial or fungal growth after 21 days |
| Viral Testing | None detected |
| Viability | ≥80% viability |
| Functional Tests | Exhibit differentiation potential of NPCs into Tuj1+ early neurons and TH+ dopaminergic neurons |
| Passage Number | P4 |
| Population Doubling Capacity | ≥15 PDLs |
| Population Doubling Time | ~38 hours |
| Name of Depositor | ATCC |
|---|---|
| Year of Origin | 2015 |
| References |
Malik N, et al. Compounds with species and cell type specific toxicity identified in a 2000 compound drug screen of neural stem cells and rat mixed cortical neurons. Neurotoxicology 45: 192-200, 2014. PubMed: 25454721 |