The Seminar series focused on how IHC and FISH have been used widely in research and diagnostics for detecting proteins and DNA, respectively, yet RNA ISH has only found limited use due to lack of sensitive and easy-to-use technologies. Next generation RNA ISH technologies now enable easy visualization of gene expression at single-molecule sensitivity and single-base resolution, both in individual cells and within the context of complex tissues. Next generation RNA ISH has been employed in ~800 high quality publications to date, indicating broad utilities in cancer, neurosciences, infectious disease, and stem cell biology research. Examples showcasing the unique applications of RNA ISH in research and diagnostics will be presented.
After the last seminar, we sat down with Dr Yuling and a video camera and asked him the top 20 question asked during the seminar series.