FAQs - RNA In Situ Hybridization Technologies For Research and Diagnostics
In May, In Vitro Technologies hosted a seminar series on Next Generation RNA In Situ Hybridization Technologies For Research and Diagnostics with the President of Advanced Cell Diagnostics, Dr Yuling Luo.The Seminar series focused on how IHC and FISH have been used widely in research and diagnostics for detecting proteins and DNA, respectively, yet RNA ISH has only found limited use due to lack of sensitive and easy-to-use technologies. Next generation RNA ISH technologies now enable easy visualization of gene expression at single-molecule sensitivity and single-base resolution, both in individual cells and within the context of complex tissues. Next generation RNA ISH has been employed in ~800 high quality publications to date, indicating broad utilities in cancer, neurosciences, infectious disease, and stem cell biology research. Examples showcasing the unique applications of RNA ISH in research and diagnostics will be presented.
And the Winner is.....
Dr Roy Kong from the Centre for Eye Research
University of Melbourne
* Discount is only up to $5,000 in value and must be spent by 31/12/17
Didn’t win the lucky door prize?
After the last seminar, we sat down with Dr Yuling and a video camera and asked him the top 20 question asked during the seminar series.
Top 20 FAQs
- Brief over view of the technology
- How many samples can be done in 1 kit and probe order?
- What is the sensitivity of the assay?
- Do you need to optimise the protocol for RNAscope®?
- Is RNAscope more stable in a tissue block or slides?
- I have very high background and can see dots that are not the actual signal – what would be causing this?
- What thickness of tissues can we use?
- Regarding RNA degradation, can samples be used which are, for example, more than 10 year old?
- Do you have to run + and – controls every time?
- Why are you comparing RNA to protein and making a comparison with them? In a nice way, aren’t you comparing apples to oranges?
- Is the RNAscope® system limited to tissue samples?
- Do any customers use RNAscope®e with Flow Cytometry?
- Can the technology be used for micro RNA samples?
- Can the technology be used to distinguish different isoforms of the same protein?
- Can you multiplex for fluorescence with a fluorecently labelled antibody and RNAScope®?
- How many different probes can you use for fluorescent multiplexing?
- Can you do IHC before or after RNAScope®?
- Is there Protein degradation issues when performing for Dual ISH-IHC?
- Is this technology becoming useful in translational research?
- Do you need to buy software to view your results?