Turnkey Solution for Rapid, Real-Time Detection of Human T Cell Activation
Immuno-metabolism is well understood to influence many aspects of immune cell response as both an indicator and controller of immune cell fate, function, and fitness.
This concept is clearly illustrated in the metabolic ramping of naive T cells to proliferate following activation signalling, and distinct metabolic phenotypes that drive effector and memory function and T cell persistence.
The Agilent Seahorse XF Hu T Cell Activation Assay is an optimized turnkey solution that detects T cell activation in minutes, providing a kinetic readout of T cell activation dynamics in real-time. This delivers a unique, early window of T cell biology, enabling researchers to quantify activation kinetics and profile modulators of T cell behavior.
Understand and control T cell activation, differentiation, and proliferation
Glucose metabolism is recognized as a driver for T cell fate and function. A rapid increase in glycolysis is a signature early event during T cell activation and is required for subsequent T cell proliferation and differentiation. By measuring the increase in glycolysis as proton efflux rate (PER), Agilent Seahorse technology provides an early kinetic window to interrogate T cell activation.
- Detect responses in minutes upon stimulation, providing an early window for studying T cell activation.
- Deliver kinetic readout for real time assessment of T cell activation dynamics, providing a comprehensive view of the activation.
Modulate T cells with integrated reagent injection ports. Monitor responses in real time.
Agilent Seahorse XF sensor cartridges incorporate integrated injection ports that sequentially deliver up to two reagents prior to the activator injection, allowing analysis of the acute effects of modulators on T cell activation.
Dive deeper with Seahorse XF cell metabolism assays to explore programs that drive T cell development and function, investigate metabolic poise and switches that are associated with T cell proliferation, differentiation and fitness, and gain new insights for immunotherapy discovery
- XF Real Time ATP Rate Assay - Metabolic reprogramming and phenotype switching can be quantitatively assessed, differentiating glycolytic and mitochondrial ATP production rate throughout the T cell life cycle.
Click here to view flyer - XF Cell Mito Stress Test - T cell persistence and fitness are tightly linked to mitochondrial function. This assay is the most comprehensive functional analysis of mitochondrial function.
Click here for more information - XF Substrate Oxidation Tests - These tests can be used with various types of immune cells to investigate substrate dependence or reliance on one or more substrates for correct immune cell differentiation and/or function.
Click here to view flyer - XF Glycolytic Rate Assay - T cell activation is associated with upregulation of glycolysis occurring from minutes to days. The glycolysis critical for the proliferation and differentiation to effector T cells can be quantitively investigated throughout the T cell life cycle.
Click here to view flyer
Podcast: Expediting cell and gene therapy workflows
Sponsored by: Cell Culture Dish
Duration: 26:37
Guests:
Dave Eansor
Bio-Techne
President, Protein Sciences Segment
Sean Kevlahan, Ph.D.
Bio-Techne
Senior Director, Cell and Gene Therapy
Featured products:
- Cloudz – Enables activation and expansion of T cells for autologous or allogeneic adoptive T cell therapy workflows such as CAR T or TCR.
- Ella – an automated platform for performing ELISAs developed by Protein Simple. Can be used for a variety of applications including in-process and QC ELISAs.
- GMP small molecules – used in iPSC derived cell therapies. Bio-Techne is the only company providing cGMP quality for therapeutic manufacturing.
- GMP proteins and cytokines - for the cell culture step in cell therapy and gene therapy.
