Janelia Fluor Dyes
Originally developed at the Janelia Research Campus, the Janelia Fluor® dyes have recently been made commercially available conjugated to antibodies or supplied as NHS esters for custom labeling. These bright, photostable dyes enable sensitive detection of cellular targets in IHC, ICC, and flow cytometry applications. They are ideal for Super-Resolution Microscopy (SRM) and are especially suited for live-cell imaging.
Janelia Fluor® Dye Key Features and Applications
- Exceptionally bright, highly photostable
- Cell permeable
- Especially well-suited to live-cell imaging
- Supplied with a choice of reactive groups for simple biomolecule conjugation
- Can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®
- Suitable for use in confocal microscopy, IHC, ICC, and flow cytometry
- Ideal for super-resolution techniques including STED and dSTORM
- Photoactivatable Janelia Fluor® dyes compatible with PALM microscopy
Cardiac Tissue labelled with Janelia Fluor® Dyes. Images acquired using dSTORM
Specifications
Spectra Viewer
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Hear exclusively from the inventor of Janelia Fluor® Dyes
Luke Lavis: Bright Ideas in Fluorescence Imaging
In this episode of the Back of the Napkin podcast, discover how the work of Luke Lavis, at the Janelia Research Campus, has pushed the boundaries of fluorescence imaging. His development of Janelia Fluor® dyes has significantly improved live-cell and super-resolution imaging capabilities.
This podcast reveals the intricate balance between fundamental research, application in drug discovery, and the collaborative culture at Janelia, emphasizing the importance of mentorship and interdisciplinary interactions.
Janelia Fluor® Dyes for Super-Resolution Microscopy
Super-resolution microscopy (SRM) techniques such as STED, STORM, dSTORM, PALM and FPALM provide far greater resolution images compared to traditional light microscopy. This is because unlike conventional light microscopy, super-resolution microscopy is not limited by the optical diffraction limit ~ 200 nm (known as the Abbe diffraction limit).
Janelia Fluor® Dyes are well suited for super-resolution techniques including STED, PALM, and dSTORM.
They are supplied with a range of reactive groups for biomolecular conjugation, including:
- Amine-Reactive
- Click-Reactive (azide and tetrazine)
- Free acid
- Sulfhydryl-Reactive (Maleimide)
Janelia Fluor® Reactive Dyes Portfolio
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Photoactivatable Janelia Fluor® Dyes
A favourable alternative to photoactivatable proteins for PALM microscopy.
PA Janelia Fluor® 646, SE and PA Janelia Fluor® 549, SE can be used on both lived and fixed cells and can be multiplexed to perform two-colour sptPALM in live cells.
Individually these dyes are suitable for single molecule tracking and PALM and sptPALM.
Spontaneously Blinking Janelia Fluor® Dyes
Allow facile single-molecule localization microscopy (SMLM) in cells and dense biomolecular structures, without the need for photoactivation or redox buffers.
These spontaneously blinking dyes harness Janelia Fluor® technology to delivery dyes that automatically cycle between 'off' and 'on' states with an ideal duty cycle for super resolution microscopy experiments.