Bio-techne: Synthetic Hydrogel
Enhance Organoid Reproducibility With Cultrex Synthetic Hydrogel
Cultrex Synthetic Hydrogel is a fully defined, synthetically derived extracellular matrix designed to improve consistency in 3D cell culture and organoid workflows.
Cultrex Synthetic Hydrogel delivers comparable performance to basement membrane extracts while reducing the inherent variability of animal-derived component formulations, providing a reliable foundation for advanced 3D cell culture systems and a path to the clinic for cell therapy applications
Why Use Synthetic Hydrogel?
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Improved Culture Consistency Fully defined composition, enabling improved testing condition reproducibility for more consistent cultures, batch after batch. |
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Uncompromised Performance Delivers organoid growth and viability comparable to animal-derived BME matrices, supporting robust cell culture systems. |
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Improved Imaging Optimized optical clarity and compatibility with brightfield and fluorescence microscopy techniques for improved monitoring and imaging. |
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Efficient Harvesting Improved matrix metalloproteinase binding enables protease digestion for more efficient harvests. |
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User-Friendly Format Supplied as a convenient, ready-to-use kit and validated protocol. For unique applications, end users are welcome to further evaluate, optimize, and validate for their process needs. |
Defined Matrix and Wnt Agonists for Human Intestinal Organoid Culture
Wnt/R-Spondin (RSPO) agonists and a defined, animal-free synthetic matrix were evaluated as alternatives to traditional components used in human intestinal organoid culture.
Key findings:
- Wnt/RSPO1-3 agonists support enteroid organoid culture.
- Wnt/RSPO1-2 agonists demonstrate higher bioactivity and drive greater organoid growth at lower concentrations.
- Cultrex Synthetic Hydrogel supports robust enteroid organoid expansion and differentiation with Wnt/RSPO2 agonist.
Synthetic Hydrogel Data
Figure 1. Organoids expanded in the Cultrex Synthetic Hydrogel exhibit expected cystic morphology, indicative of healthy baseline development. Upon differentiation, the organoids begin to lose their cystic morphology and start forming more specialized, structured tissues. This demonstrates Cultrex Synthetic Hydrogel's ability to support both healthy initial organoid growth and subsequent differentiation into mature structures.
Figure 2. Cultrex UltiMatrix BME domes were plated at 10,000 cell per 10μL hydrogel and Cultrex Synthetic Hydrogel domes were plated at 15,000 cells. After three days in culture, comparable organoid growth is observed.
Figure 3. Domes using animal derived matrix from another supplier and Cultrex Synthetic Hydrogel were each plated at 10,000 cell per 10μL hydrogel. Morphologically similar organoid growth is observed after 4 and 5.5 days, respectively.
Figure 4. Successful organoid growth observed in Cultrex Synthetic Hydrogel while organoids encapsulated in a competitor's defined hydrogel showed failed organoid formation and significant cell death by Day 4.
