RNAscope ISH technology
Although in situ RNA detection techniques (ISH and FISH) have been around for years, they are largely ineffective. They lack robustness and sensitivity to reliably detect the expression of most human genes. Microarray and PCR are gold standard molecular methods for disease profiling, but clinically relevant information regarding cellular and tissue context, as well as spatial variation of the expression patterns, is lost in the process.
Inspired to solve these challenges, Scientists at Advanced Cell Diagnostics revolutionized the RNA in situ hybridization method and in 2011 introduced RNAscope ISH for detection of target RNA within intact cells. The assay represents a major advance in RNA ISH approaches, with its proprietary probe design that simultaneously amplify target-specific signals and suppress background noise from non-specific hybridization. Known for its sensitivity and specificity, RNAscope ISH is now a proven pioneering technology in the advancing field of spatial genomics, featured in over 9,000 publications across a multitude of research areas.
Get started or establish the RNAscope Technology in your lab with a 20% discount on HybEZ II Hybridization system when you bundle with reagents and probes worth $12,000 or more.
Please quote discount code MKT0503 to receive this offer
*Offer expires 30th June 2024. Not available in conjunction with any other offer.
This webinar provides a comprehensive overview of our RNAscope ISH assays to help new RNAscope users set up and get started.
We discuss the workflows for each manual assay, including required materials and reagents, tips for sample preparation and pre-treatment optimization, and guidelines for image acquisition and data analysis. We also discuss recommendations for troubleshooting and optimization.
Learning Objectives:
- Overview of RNAscope ISH Technology and assays.
- How to get started and best practices.
- Assay workflows and sample pre-treatment recommendations.
- Tips and tricks for a successful assay.
- Guidelines for image processing and data analysis.
- Troubleshooting and optimization tips.
Speaker
Eirini Vagena, PhD
Technical Support Scientist
ACD, a Bio-Techne Brand
Any gene, any species, any tissue.
