RNAscope™ Assay

Flexible solutions for all your research needs

Independent of your genes of interest, our easy RNA in situ hybridization assay uses common reagents and protocols; providing universal assay conditions. All RNA-ISH kits contain reagents in a convenient ready-to-use (RTU) format, and chromogenic dyes are available to interrogate a single or two genes simultaneously. Most versatile are our RNAscope™ Multiplex Fluorescent Reagent Kits, enabling you to interrogate 1, 2 or 3 genes simultaneously.

Automated RNAscope™ ISH assays are also available for Leica Systems (LS) and Roche [Ventana] Systems (VS).

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> RNAscope™ 2.5 HD Assay - BROWN
> RNAscope™ 2.5 HD Assay - RED
> RNAscope™ 2.5 HD Duplex Assay

The RNAscope 2.5 High Definition (HD)—BROWN Assay, is based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope Assays do not require the RNA-free environment used for traditional ISH. The HD version is the most sensitive RNA ISH method and can be used for low expressing gene targets.

RNAscope 2.5 HD Reagent Kit—BROWN is the ideal starter kit for first time users. This very robust assay gives reliable results which are ideal for archiving due to the permanent staining. It is the standard in molecular pathology diagnostics, usable for a wide source of samples and the results are visible under any standard bright field microscope. The RNAscope 2.5 HD - Brown Reagent Kits are designed to provide more intense Diaminobenzidine (DAB) staining when low copy target gene expression is anticipated (1–20 copies per cell). The RNAscope 2.5 HD reagent kits are compatible with the same RNAscope probes used previously with RNAscope 2.5 assays.

The RNAscope 2.5 HD—BROWN Manual Assay Workflow Requires:

• RNAscope Target Probes (Catalog or Made-to-Order channel 1 (C1) Probes)
• RNAscope Control Probes (Select species-specific positive control probes and negative control probes)
• RNAscope Control Slides
• RNAscope 2.5 HD Reagent Kit—BROWN (Pretreatment Kit, Detection kit, and Wash Buffer included)
• Brightfield microscope
• HybEZ™ Hybridization System

The RNAscope 2.5 High Definition (HD)—Red Assay, is based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides. RNAscope Assays do not require the RNA-free environment used for traditional ISH. The HD version is the most sensitive RNA ISH method and can be used for low expressing gene targets.

The RNAscope 2.5 HD Reagent Kit-RED assay with it Fast Red dye offers a higher contrast and is the first choice in situ hybridization applications where chromogenic staining with DAB is less desirable, such as staining of highly pigmented lung, liver, retina and melanoma tissue specimens. Also for genes where a lower expression is assumed ACD recommends this assay as the red dots clearly distinguish against the hematoxylin staining and are visible under are standard brightfield microscope.

The RNAscope 2.5 HD--RED Manual Assay Workflow Requires:

• RNAscope Target Probes (Catalog or Made-to-Order C1 Probes)
• RNAscope Control Probes (Select species-specific positive control probes and negative control probes)
• RNAscope Control Slides (optional)
• RNAscope 2.5 HD Reagent Kit—RED (Pretreatment Kit, Detection kit, and Wash Buffer included)
• Brightfield microscope or multispectral fluorescent imaging system
• HybEZ™ Hybridization System