Molecular Resources
Nucleic Acid Purification
DNA and RNA Purification Product Selector
To find the appropriate nucleic acid purification product for your application, filter by the radio button items on the left side. If you have a question about a particular sample type that is not included in this selector, please contact Technical Services to find the solution that is right for you.
New to purifying nucleic acid? Promega offer a complete guide to the basics of DNA isolation, plasmid growth and DNA quantitation. Get up to speed with the DNA Purification Guide!
Get to know all of the various methods for studying gene expression and tips for isolating and working with RNA with Promega’s Gene Expression Analysis guide. Understand the in’s and out’s before you begin with Promega.
Looking to understand Forensics? Promega can guide you through DNA analysis in human identification applications (like forensic casework and relationship testing).
Cell Health
Not sure which way to go when studying apoptosis? Understand the molecular mechanisms that confirm early or late stage apoptosis, and find out more about the benefits of using multiplexed assays with a single sample in Promega’s Apoptosis Guide
Describes various cell viability assays and how to choose the right assay for your needs.
PCR and qPCR
Restriction Enzyme Tool
Search for restriction enzymes by name, recognition sequence or overhang. Explore compatible enzymes for double digests quickly, without having to select each individually.
Real-time quantitative PCR
Real-time quantitative PCR, or qPCR, has become a standard tool in the analysis of nucleic acids. By incorporating fluorescently labeled probes or nucleic acids or fluorescent double-stranded DNA-binding dyes into the PCR reaction, product formation can be monitored following each PCR cycle. Since product formation is detected in the exponential phase of PCR where the reaction is most efficient, quantification of starting material is more accurate and sensitive. Most common qPCR detection methods include probe-based as well as dye-based detection chemistries. Learn more about each type of method and the proven systems we offer.
Custom PCR Protocols
Thinking (and working) outside the standard PCR box? Create custom PCR protocols in seconds (standard, hot start or qPCR) that you can print or share easily with colleagues. Simply choose the type of PCR you want to use, enter the custom parameters for your experiment and press 'View Protocol'. All of the calculations will be performed for you and the new protocol will be displayed. Save time writing out detailed protocols with this handy tool from Promega.
Flexi Vector Design Tool
> The Flexi Vector Primer Design Tool will design PCR primers for use with Flexi® Cloning System Vectors which will amplify a compatible coding region from your input sequence.
> When you launch the Flexi Vector Primer Design Tool it will open in a new browser window. When finished with the tool, please close the window and continue from this page.
Background of basic cloning with a focus on cloning PCR fragments.
Protein
Want to go cell-free with your protein expression but you’re not sure where to start? Learn the basics of in vitro transcription and translation and cell-free protein expression methods with Promega’s guide to protein expression.
Investigating a protein? If you need to purify it for further analysisbut aren’t sure where to start then try Promega’s guide to protein purification and analysis. A comprehensive introduction to protein purification and analysis methods protocols and references to help you get up and running quicker!
Gene Expression
Find my Gene
Find My Gene is an online gene exploration tool that integrates a number of functionalities to help researchers better understand their gene's function, such as:
> Search for genes by blasting DNA, RNA, or amino acid sequences.
> Learn detailed information about a gene of interest.
> Explore literature-validated protein interactions.
> Find relevant products specific to a gene of interest, including expression-validated ORF clones.
Various methods for studying gene expression and tips for isolating and working with RNA.
Cell Analysis
The Transfection Assistant helps you optimize transfection protocols using information from the published literature. It provides information on transfection of specific cell types, including the amount of nucleic acid and ratio of reagent:nucleic acid used for specific plate formats, and provides links to the primary literature.
Select a cell line and transfection reagent to search the database or select “View All” to see the entire database contents.
Molecular basis of cell signalling and the various assays used to study cell signalling.
Protocols for transfection and information on optimization.