The Seahorse XFe96 Analyzer measures OCR and ECAR of live cells in a 96-well plate format. These rates are key indicators of mitochondrial respiration and glycolysis and provide a systems-level view of cellular metabolic function in cultured cells and ex-vivo samples.

Features

• Real-time Results- this integrated system reports metabolic rates in just minutes, without sample extraction or labeling. Wave software controls the instrument and performs rate measurements on the fly for same day results.
• Live-Cell Responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing while maintaining physiologic temperature (37oC).
• Flexible Assay Design- the 96-well plate format accommodates many conditions in a single run and is best for dose-response studies and compound screening.
• High Sensitivity- analyze as few as 5000 cells per well in the custom 96-well plate. Cell number requirements vary by cell type; consult the Cell Reference Database for details.
• Measure mitochondrial function with the Seahorse XF Cell Mito Stress Test
• Generate a metabolic phenotype within one hour with the Seahorse XF Cell Energy Phenotype Test
• Determine the ability of cells to use the glycolytic pathway to meet energy demand with the Seahorse XF Glycolysis Stress Test
• Quickly determine dependency of cellular energy production on mitochondrial substrates with the Seahorse XF Mito Fuel Flex Test
• Analyze 3D samples such as spheroids and islets with the Seahorse XFe96 Spheroid Plate
• Easily create assay protocols and analyze data with Seahorse Wave software
• For Research Use Only. Not for use in diagnostic procedures. 

Literature

• Seahorse XFe Analyzers

Enhance your research with a 24- or 96-well format instrument

• Investigating Warburg metabolism in cancer cells using the Seahorse XF Mito Fuel Flex Test