MTT can be used to safely assess cell proliferation, cell viability, and/or cytotoxicity. MTT is added directly to the culture medium and is reduced by metabolically active cells to insoluble purple formazan dye crystals. The absorbance of the sample is read directly in the wells at an optimal wavelength of 570 nm, but any filter that absorbs between 550 and 600 nm may be used.
Label: Tetrazolium compound MTT changes to from yellow to purple
Testing Format: Microplate reader
Sample Type: Cultured cells
Size: 50 x 96-well microplates