In Vitro Technologies :: Products

Permits and Restrictions	View Permits
Organism	Homo sapiens, human
Tissue	colon
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	normal
Age	21 weeks gestation fetus
Gender	female
Storage Conditions	liquid nitrogen vapor phase

Karyotype	The line is diploid and no consistent marker chromosomes were observed.
Images
Clinical Data	female fetus 21 weeks gestation
Comments	Morphologically the cells resemble epithelial cells; however, the cells do not contain keratin and definitive evidence of epithelial origin is lacking.

Complete Growth Medium	The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Volumes are given for a 75 cm² flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes. Discard supernatant. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Twice per week
Cryopreservation	Freeze medium: complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO₂), 5%

STR Profile	Amelogenin: X CSF1PO: 10,11 D13S317: 11,13 D16S539: 10,11 D5S818: 12,13 D7S820: 11 THO1: 7,8 TPOX: 9,10 vWA: 14,18

Name of Depositor	A Thompson
References	J. Tissue Culture Methods 9: 117-122, 1985.

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