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Biotechne has been extensively involved in formulating a basement membrane extract for 3D cell culture.
NEW Cultrex UltiMatrix RGF BME is specially formulated with higher extracellular protein content, consistency, and clarity for optimal organoid, spheroid, and pluripotent stem cell culture.
Watch below how Cultrex UltiMatrix RGF BME is used to grow Human Intestinal Organoids (MUC2 (magenta) Expression in Human Intestinal Organoids)
• Consistent lot-to-lot performance
• Qualified for organoid and pluripotent stem cell culture
• Tensile strength optimized for 3D applications
• Verified for dome formation
Cultrex BMEs are a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. These extracts provide a natural extracellular matrix hydrogel that polymerizes at 37 °C to form a reconstituted basement membrane that is used to promote and maintain a differentiated phenotype of cell cultures such as epithelial cells, endothelial cells, glandular cells, neurons, and smooth muscle cells. BME can also be utilized in cell attachment, neurite outgrowth, angiogenesis, in vitro cell invasion and in vivo tumorigenicity assays.
• Rapid - gels within 30 minutes at 37 °C.
• Reliable - maintains gelled form in culture medium for 14 days.
• Sterile - screened for bacteria, fungi, and mycoplasma.
• Specific - available with reduced growth factor content, with or without Phenol Red, and additional sterility testing.
|Application||Name||Description||RGF Version Available?||Notes|
|Organoids and Stem Cells||NEW! Cultrex UltiMatrix RGF BME||Soluble form of basement membrane that provides high tensile strength, enhanced levels of entactin/nidogen, elevated protein concentration, and robust clarity and purity.||Yes||This matrix contains compositional enhancements and performance benefits for organoid and stem cell culture.|
|Stem Cells||Cultrex Stem Cell Qualified BME||Provides an effective feeder-free surface for the attachment and maintenance of stem cells in an undifferentiated state, enabling growth promotion or study of stem cell differentiation.||Yes||Attachment and maintenance of stem human embryonic and induced pluripotent stem cells.|
|Stem Cells||Cultrex ReadyBME, Stem Cell Qualified||Pre-diluted, pre-qualified ready-to-use basement membrane extract for feeder-free pluripotent stem cell maintenance and differentiation.||Yes||Improves experiment consistency and success by minimizing user handling and coating matrix dilution variability.|
|3-D Tissue Spheroids||Cultrex 3-D Culture Matrix BME*||BME used for modeling the morphological effects of early oncogenesis on 3-D microenvironments, wherein healthy, differentiating cells exhibit a structured, polarized morphology that is critical for tissue formation and function. Specifically produced for and qualified in 3-D culture studies.||Yes||3-D cell cultures.|
|Organoid||Cultrex Organoid Qualified BME, Type 2||A Reduced Growth Factor BME that has been developed and qualified specifically for robust organoid cultures.||Yes||Robust organoid cultures.|
|Organoid||Cultrex Organoid Qualified BME, Type R1||A Reduced Growth Factor BME that has been developed and qualified specifically for difficult organoid cultures.||Yes||Difficult to culture organoids.|
|Cancer/Tumorspheres||Cultrex BME, Type 3*||BME Type 3 helps cells to adapt to in vivo transplantation. Mimicking the in vivo microenvironment, including low glucose and low pH, to improve take rate and growth of implanted cells for xenograft and tumorgraft models.||No||Xenograft/Tumorgrafts.|
|General||Cultrex BME*||Consists of laminin I, type IV collagen, entactin, and heparan sulfate proteoglycan.||Yes||General use including 2-D cultures, 3-D cultures, cell attachment and cell invasion.|