Single Cell Isolation Methods for Cell line Development

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Bio-Techne's single cell dispensers offer an automated, high-throughput alternative to traditional single cell isolation methods such as FACS sorting.

When using a Bio-Techne single cell dispenser in your cell line development workflow, CHO cells are transfected with a plasmid, encoding the desired protein for mass production, as for traditional methods. Following transfection, however, cells in suspension are loaded into a proprietary microfluidic single cell or bulk sorting cartridge and dispensed into 96- or 384-well plates using our Pala Single and Bulk Cell Sorter. A single cell cloning protocol is then used to generate cell colonies, and the resulting colonies can be analyzed via ELISA to determine antibody titer.

The rapid and gentle single cell isolation offered by our benchtop instruments can help to preserve cell integrity and viability.

Bio-Techne’s single cell sorting technology yields higher cloning efficiency and better scalability.

Fast Cell Isolation and Single Cell Dispensing
Empower your cell line development workflow with the fastest and simplest way to identify and isolate single cells in as little as 7 minutes from instrument start-up!

Efficient Single Cell Sorting
Proprietary benchtop sorting technology is affordable, user-friendly, and requires minimal training.

Gentle Cell Sorting and Dispensing
Cell samples are sorted and dispensed under gentle conditions (<2 PSI) helping to preserve viability and integrity throughout the cell line development workflow.

The figure (right) shows that Bio-Techne instruments are highly efficient and consistent at dispensing single cells (80-90% with mammalian cells) compared to manual pipetting (~30%).