Novus Polarity Sensitive Indicator of Viability Flow Cytometry Kit, 100 Tests Item ID: NOVNBP229611100Tests

Publications for Polarity Sensitive Indicator of Viability Kit (NBP2-29611)(13)

We have publications tested in 2 confirmed species: Human, Mouse.We have publications tested in 3 applications: FLOW, Flow-CS, ICC/IF. Submit a Publication Filter By Application FLOW (2) Flow-CS (3) ICC/IF (1) All Applications Filter By Species Human (2) Mouse (5) All Species Showing Publications 1 - 10 of 13. Show All 13 Publications. Collapse Publications.

Publications using NBP2-29611	Applications	Species
Sommer A, Kordowski F, Buch J et al. Sommer A, Kordowski F, Buch J et al. Phosphatidylserine exposure is required for ADAM17 sheddase function. Nat Commun. May 10 2016 12:00AM [PMID: 27161080] (FLOW, Human) Nat Commun. May 10 2016 12:00AM [PMID:27161080] (FLOW, Human)	FLOW	Human
Lew ED, Oh J, Burrola PG et al. Lew ED, Oh J, Burrola PG et al. Differential TAM receptor-ligand-phospholipid interactions delimit differential TAM bioactivities. Elife. 2014 Oct 24 [PMID: 25265470] (FLOW, Mouse)Details:Polarity Sensitive Indicator of Viability Flow Assay Kit used for quantifying apoptosis in adherent cells or supernatant cells from Axl_TAM TKO MEF cultures - cells stained with propidium iodide which bind dead cells and pSIVA which fluoresces when bound to PtdSer (data in Figure 4B). Elife. 2014 Oct 24 [PMID:25265470] (FLOW, Mouse)	FLOW	Mouse
Pradhan N, Pratheek BM, Garai A et al. Pradhan N, Pratheek BM, Garai A et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells. Cell Biol. Int. 2014 May 07 [PMID: 24804954] (Flow-CS, Human, Mouse)Details:Fig 4: mouse splenocytes, EL4 mouse lymphoma cells, human PBMC, Jurkat human T cells; Fig 5: Jurkat. Cell Biol. Int. 2014 May 07 [PMID:24804954] (Flow-CS, Human, Mouse)	Flow-CS	Human, Mouse
Nagaria TS, Williams JL, Leduc C et al. Nagaria TS, Williams JL, Leduc C et al. Flavopiridol synergizes with sorafenib to induce cytotoxicity and potentiate antitumorigenic activity in EGFR/HER-2 and mutant RAS/RAF breast cancer model systems. Neoplasia. 2013 Aug [PMID: 23908594] (Flow-CS)Details:Flow cytometry (Cell surface): MDA-MB-231 (Fig 4A) and MDA-MB-468 (Fig 4B) adenocarcinoma cells. Neoplasia. 2013 Aug [PMID:23908594] (Flow-CS)	Flow-CS
Suhane S, Kanzaki H, Arumugaswami V et al. Suhane S, Kanzaki H, Arumugaswami V et al. Mitochondrial NDUFS3 regulates the ROS-mediated onset of metabolic switch in transformed cells. Biol Open. 2013 Mar 15 [PMID: 23519235] (Flow-CS)Details:pSIVA-IANBD Flow Kit: Flow (Cell Surface): Fig 1 (HEK293 cells). pSIVA-IANBD was used to determine the basal level of apoptosis in HEK cells. Biol Open. 2013 Mar 15 [PMID:23519235] (Flow-CS)	Flow-CS
Demchenko AP. Demchenko AP. The change of cellular membranes on apoptosis: fluorescence detection. Exp Oncol. 2012 Oct [PMID: 23070011]Details:Live imaging: pSIVA as an important advancement in annexin based methodology. Exp Oncol. 2012 Oct [PMID:23070011]
Ruggiero L, Connor MP, Chen J et al. Ruggiero L, Connor MP, Chen J et al. Diurnal, localized exposure of phosphatidylserine by rod outer segment tips in wild-type but not Itgb5-/- or Mfge8-/- mouse retina. Proc Natl Acad Sci U S A. 2012 May 22 [PMID: 22566632] (Mouse)Details:Live tissue imaging (mouse retina): Figs 4, 5. S4. pSIVA-IANBD was added to dissected live mouse retina and shown to label the tips of photoreceptor outer segments (POS). The results suggested that phosphatidylserine (PS) exposure is specific to the POS surface. Furthermore, enhanced PS exposure preceded rod shedding and phagocytosis, suggesting that surface PS exposure promotes these processes. Proc Natl Acad Sci U S A. 2012 May 22 [PMID:22566632] (Mouse)		Mouse
Krajewska M, You Z, Rong J et al. Krajewska M, You Z, Rong J et al. Neuronal deletion of caspase 8 protects against brain injury in mouse models of controlled cortical impact and kainic acid-induced excitotoxicity. PLoS One. 2011 [PMID: 21957448] (Mouse)Details:IF (mouse primary neuron cultures): Figs 2B, 3. Cells were labeled with pSIVA-IANBD for 15 min, fixed and stained with a MAP2 antibody and DAPI. Cells double-stained with both pSIVA-IANBD and MAP2 (neuronal marker) were identified as degenerating neurons. PLoS One. 2011 [PMID:21957448] (Mouse)		Mouse
Graewe S, Rankin KE, Lehmann C et al. Graewe S, Rankin KE, Lehmann C et al. Hostile takeover by Plasmodium: reorganization of parasite and host cell membranes during liver stage egress. PLoS Pathog. 2011 Sep [PMID: 21909271] (ICC/IF)Details:IF (HepG2 cells infected with P. berghei parasites), Fig 1 PLoS Pathog. 2011 Sep [PMID:21909271] (ICC/IF)	ICC/IF
Warnes G, Martins S. Warnes G, Martins S. Real-time flow cytometry for the kinetic analysis of oncosis. Cytometry A. 2011 Mar [PMID: 21254392]Details:Live imaging: pSIVA as an assay for real-time detection of apoptosis. Cytometry A. 2011 Mar [PMID:21254392]
Zhang QC, Yeh TL, Leyva A et al. Zhang QC, Yeh TL, Leyva A et al. A compact beta model of huntingtin toxicity. J Biol Chem. 2011 Mar 11 [PMID: 21209075] (Mouse)Details:A pSIVA-IANBD based cell suspension toxicity assay was used to determine cell viability in mouse Neuro2A (neuroblastoma) overexpressing huntingtin proteins (Fig 4). J Biol Chem. 2011 Mar 11 [PMID:21209075] (Mouse)		Mouse
Skommer J, Darzynkiewicz Z, Wlodkowic D. Skommer J, Darzynkiewicz Z, Wlodkowic D. Cell death goes LIVE: technological advances in real-time tracking of cell death. Cell Cycle. 2010 Jun 15 [PMID: 20519963]Details:Live cell imaging (etoposide treated cell lines & NGF-deprived primary neuronal cultures): Discussion about tools for tracking cell death real-time. Cell Cycle. 2010 Jun 15 [PMID:20519963]
Kim YE, Chen J, Chan JR et al. Kim YE, Chen J, Chan JR et al. Engineering a polarity-sensitive biosensor for time-lapse imaging of apoptotic processes and degeneration. Nat Methods. 2010 Jan [PMID: 19966809]Details:Real-time live-cell imaging and time-lapse microscopy of apoptosis: Fig 2 (Cos-7 cells), Fig 3 (neuronal degeneration), Fig 4 (axonal degeneration), Fig 5 (rescue of neuronal degeneration as visualized by pSIVA). Nat Methods. 2010 Jan [PMID:19966809]
Show All 13 Publications. Collapse Publications.

Reviews for Polarity Sensitive Indicator of Viability Kit (NBP2-29611) (1) 41

Average Rating: 4 (Based on 1 reviews) We have 1 review tested in 1 species: Human. We have 1 review tested in 1 application: Flow. Submit a Review Reviews using NBP2-29611: Filter by Applications Flow (1) All Applications Filter by Species Human (1) All Species

Images	Ratings	Applications	Species	Date	Details
Â	reviewed by:Anonymous	Flow	Human	06/08/2016	View Summary Application Flow Cytometry Sample Tested CEM T cell line Species Human Lot 100313 Blocking Blocking Details none Primary Anitbody Dilution Ratio use product at 1/50 in HBSS, analyze cells after 5- 30 minutes, keep cells dark and at room temperature, do not fix cells Details Detection Notes BD accuri flow, use non irradiated cells as negative control Fixation Details spin cells at 1000 rpm for 2 min, resuspend in HBSS Permeabilization Details none Wash Description none Comments Comments important: this flow reagent does not work for microscopy. use microscopy pSIVA kit instead (NBP2-29382)

Product General Protocols

View specific protocols for Polarity Sensitive Indicator of Viability Kit (NBP2-29611):

• MSDS (NBP2-29611)

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

• Flow Cytometry
• View all Protocols, Troubleshooting, Illustrated assays and Webinars.

FAQs for Polarity Sensitive Indicator of Viability Kit (NBP2-29611). (Showing 1 - 10 of 18 FAQs).

1. What are the applications of pSIVAâ„¢-IANBD?
   • pSIVAâ„¢-IANBD is ideal for live imaging microscopy, confocal microscopy, fluorescence microscopy, in vivo applications and flow cytometry.
2. How does pSIVAâ„¢-IANBD work?
   • pSIVAâ„¢-IANBD binds to exposed phosphatidylserine (PS) residues on the surface of live cells.
3. How is pSIVAâ„¢-IANBD constructed?
   • pSIVAâ„¢ is a recombinant Annexin B12 construct, and is conjugated to IANBD which is a polar sensitive dye.
4. What are the similarities between Annexin V and pSIVAâ„¢-IANBD?
   • Both Annexin V and pSIVAâ„¢-IANBD bind to exposed PS on the surfaces of cells. Annexin V-FITC is the most commonly used Annexin V conjugate. Annexin V-FITC and pSIVAâ„¢-IANBD both excite at 488 nm and emit fluorescence at 530 nm.
5. What is the biological significance of exposed PS?
   • Exposed PS is a hallmark of early apoptosis and also occurs during other cell death processes. PS exposure may also occur during homeostasis and other normal cellular events.
6. What are the differences between Annexin V fluorescent conjugates and pSIVA™™™™-IANBD?
   • Annexin V fluorescent conjugates such as Annexin V-FITC fluoresce irrespective of whether or not they are bound to PS. In contrast pSIVA-IANBD fluoresces only when bound to PS. This is because pSIVA-IANBD is polar sensitive. pSIVA-IANBD fluoresces in non-polar but not polar environments. When pSIVAâ„¢-IANBD is bound to PS it is in the non-polar environment of the membrane lipid bilayer and fluoresces. However, when pSIVA-IANBD is not bound it is in the polar environment of the media or buffer and does not fluoresce. Annexin V-FITC requires washes to remove excess, unbound fluorescent molecules. However since pSIVAâ„¢-IANBD only fluoresces when bound to PS, washing is not needed.
7. What are the advantages of using pSIVA™™™-IANBD compared to Annexin V fluorescent conjugates?
   • Annexin V conjugates are primarily useful for flow cytometry. In contrast pSIVAâ„¢-IANBD is also ideal for live imaging microscopy, confocal microscopy, and immunofluorescence microscopy. pSIVA-IANBD, but not Annexin V-FITC can be used to study reversible or transient PS exposure events. This is because when PS exposure reverts to the inner bilayer, pSIVA-IANBD will be released into the polar environment of the media of buffer and no longer fluoresce. So why can’t you use Annexin V-FITC to study this phenomenon? If Annexin V-FITC were released back into the polar environment it would still fluorescence and hence it wouldn’t be possible to track transient PS exposure events because they wouldn’t be associated with loss of fluorescence as fluorescent conjugates like Annexin V-FITC are fluorescent irrespective of whether or not they are bound to their targets.
8. Why is pSIVA™™™-IANBD but not Annexin V-FITC useful for microscopy?
   • Annexin V-FITC has too much background for microscopy applications. It fluoresces all the time and washing is required to remove unbound Annexin V-FITC. In contrast since pSIVA-IANBD fluoresces only when bound to PS background is lacking. pSIVA-IANBD can be incubated with cells and monitored by live imaging because no wash is required.
9. Has pSIVAâ„¢-IANBD been published?
   • Yes, there are a number of pSIVA-IANBD publications. Please see the publication list in the pSIVA-IANBD manual.
10. Is pSIVAâ„¢ available in other conjugates besides IANBD?
    • No, pSIVAâ„¢ is not currently available in other conjugates.
11. Can pSIVAâ„¢-IANBD labeled cells be fixed?
    • Yes, once living cells are stained with pSIVA-IANBD they can be fixed and stained with other markers for flow cytometry or fluorescence microscopy.
12. Can be pSIVAâ„¢-IANBD be used in fixed tissue or frozen tissue?
    • No, pSIVAâ„¢-IANBD is used with live cells or living, fresh tissue.
13. Is pSIVA&#8482-IANBD toxic?
    • pSIVA&#8482-IANBD is not in of itself toxic to cells in the model systems heretofore described. However, since pSIVA&#8482-IANBD binds to exposed PS, it may be possible that the binding up of exposed PS could have an unknown biological or biochemical effect in some model systems.
14. What species does pSIVAâ„¢-IANBD work in?
    • pSIVA-IANBD is not species specific and should theoretically works in any species ranging from worms to human. This pSIVAâ„¢-IANBD, like Annexin V-FITC, binds to exposed PS, which is a phenomenon from worms to humans. Theoretically, pSIVAâ„¢-IANBD should be suitable for any species that Annexin Vi-FITC has been used in.
15. How has pSIVAâ„¢-IANBD been used for in vivo applications?
    • An example would be injection into rat nerves to look at the effects on nerve injury (<a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=Nature+Methods+7%3A67-73" target="_blank">Kim et al, Nature Methods 7:67-73, 2010</a>).
16. Where can I learn more about the nitty gritty behind pSIVAâ„¢-IANBD?
    • Please refer to the two 2010 development publications by Kim et al (<a href="http://www.ncbi.nlm.nih.gov/pubmed/?term=Nature+Methods+7%3A67-73" target="_blank">Nature Methods 7:67-73, 2010</a> Protocols 5:1`396-1405).
17. Our end user is working on tissues (epithelium of imaginal disk from drosophila leg). The tissues are kept growing on a culture medium and they manage to follow apoptosis properly with orange acrylin but not with annexin V because of background. Did you ever test any of your pSIVA apoptosis kits (NBP2-29382, NBP2-29611) on tissue culture instead of cell culture ? Is there a chance the proble will work on such an application ? Would you be able to provide a sample for this application ? As for the other samples, I'm still waiting on customer feedback and I'll keep you posted.
    • Yes, pSIVA has been used in tissue applications as denoted on the publication link. Specifically, I would encourage the research to look at the following publications from the link: 1. Diurnal, localized exposure of phosphatidylserine by rod outer segment tips in wild-type but not Itgb5-/- or Mfge8-/- mouse retina. Ruggiero L, MP Connor, J Chen, R Langen, SC Finnemann. PNAS 109:8145-4148 (2012). Live tissue imaging (mouse retina): Figs 4, 5. S4. pSIVA-IANBD was added to dissected live mouse retina and shown to label the tips of photoreceptor outer segments (POS). > The results suggested that phosphatidylserine (PS) exposure is specific to the POS surface. Furthermore, enhanced PS exposure preceded rod shedding and phagocytosis, suggesting that surface PS exposure promotes these processes. 2. Diurnal photoreceptor outer segment shedding: contributions of the RPE. Finnemann SC, L Ruggiero, MP Connor. Invest Opthalmol Vis Sci 53:4327 (2012). Live Imaging: mouse retina. pSIVA was used quantify the surface exposure of phosphotidylserine on the distal rod photoreceptor outer segments. 3. Characterization of cell cycle modifications induced by electric pulses in human corneal endothelium. Thi MH, Z He, N Campolmi, S Piselli, P Gain, M Peoc'H, JM Dumolllard, S Acquart, O Garraud, G Thuret. Acta Opthalmologica 90:s249 (2012). Live tissue imaging (human cornea organ cultures): pSIVA-IANBD was used to assess cell death following gene electrotransfer into corneal endothelial cells. > 3. Engineering a polarity-sensitive biosensor for time-lapse imaging of apoptotic processess and degeneration. Kim YE, J Chen, JR Chan, R Langen. Nature Methods 7:67-73 (2010a). Real-time live-cell imaging and time-lapse microscopy of apoptosis: Fig 2 (COS-7 cells), Fig 3 (rat neuronal degeneration), Fig 4 (rat axonal degeneration), Fig 5 (rescue of rat neuronal degeneration as visualized by pSIVA. (this was also used in a rat nerve injury tissue model) Please watch the pSIVA webinar on our website for additional information about pSIVA. The webinar is referred to March 25, 2014: Real-Time Tracking of Cell Death Webinar. We would certainly be pleased to offer any of your end user research customers a sample who is willing to provide a short explanation about what they are planning to do, their timeframe, and provide feedback about how it worked for them. For any samples, please specify if you
18. Could you specify the composition of the binding buffer in the pSIVA-INABD Apoptosis/Viability Flow Kit (Cat No:NBP2-29611), because my binding buffer is often exhausted long time before the pSIVA-IANBD and propidium iodide is used up? I assume that it is a standard buffer with additional CaCl2 (like I am preparing it for micrsocopy time lapse studies with the microcopy kit (Cat No: NBP2-29382)).
    • Thank you for your inquiry about pSIVA and for using our products. Here is the requested recipe: 10X Binding Buffer: 100mM HEPES pH 7.4, 1.4M NaCl, 25mM CaCl2
Show All 18 FAQs.

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