Novus Plasmacytoid Dendritic Cell Assay Kit, 1 Kit Item ID: NOVNBP229610

Plasmacytoid Dendritic Cell Assay Kit Summary

Kit Type

Assay Kit

Packaging, Storage & Formulations

Storage

Store at 4C. Do not freeze.

Applications/Dilutions

Application Notes

Plasmacytoid Dendritic Cell (pDC)/TLR9 Kit is validated by Flow Cytometry for identifying human TLR9 expression in pDC from human whole blood, freshly isolated peripheral blood mononuclear cells (PBMC) and frozen PBMC. Before you begin: It is recommended that users follow the protocol provided for the best results with this kit. Permeabilization buffer is supplied as a 10X solution. Dilute with deionized water to its fi nal 1X working concentration immediately prior to use (example: 1 ml of 10X Permeabilization buffer to 9 ml of deionized water). Single color (AF647, FITC, PE, PerCP-Cy5.5) stained samples are recommended as compensation controls for fl ow cytometric analysis. Reagents for compensation controls are not provided in this kit. All staining and incubation steps should be done using light protected procedures. Commonly this is done by covering the sample racks or ice bucket with tin foil.

Publications

Read Publication using NBP2-29610 in the following applications: Flow-CS 1 publication Flow-IC 1 publication

Kit Components

Components

(Human CD3- CD14-CD16-CD19-CD20-CD56-HLA-DR antibodies)* 10 ul/test 300 ul Staining buffer (1X) 2 x 60 ml Permeabilization buffer (10X) 2 x 60 ml TLR9 PE (human TLR9 PE conjugate) antibody 5 ul/test 125 ul Mouse IgG1 Isotype control PE conjugate antibody 5 ul/test 125 ul Mouse IgG1 Isotype control Alexa Fluor 647 conjugate antibody 5 ul/test 125 ul Fixation buffer (1X) 60 ml hLMAX: Human Lineage Marker Antibody Mix CD123 AF647 (human CD123 Alexa Fluor 647 conjugate) antibody 5 ul/test 125 ul

Alternate Names for Plasmacytoid Dendritic Cell Assay Kit

• Human Plasmacytoid Dendritic Cell (pDC)
• Human Plasmacytoid Dendritic Cell
• Plasmacytoid Dendritic Cell (pDC)

Background

Plasmacytoid Dendritic Cells (pDC) are a subset of peripheral blood Dendritic Cells which play an important role in both innate and adaptive immune responses. pDC recognize viral components via Toll-like Receptors (TLR) including TLR7 and TLR9, and then rapidly produce Interferon-alpha (IFNalpha) as a primary anti-viral immune response. pDC also participate in antigen presentation, an important functional role which drives the adaptive immune response. Intense investigation has led to pDC identifi cation with a unique set of markers by fl ow cytometry. pDC are usually present in relatively low frequency in peripheral blood. Direct identifi cation of subsets of peripheral blood DCs has an advantage in analyzing changes in number or function of DC subsets during chronic viral infections or diseased patients, as examples.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.