Novus Plasmacytoid Dendritic Cell Assay Kit, 1 Kit Item ID: NOVNBP229609

Plasmacytoid Dendritic Cell Assay Kit Summary

Kit Type

Assay Kit

Packaging, Storage & Formulations

Storage

Store at 4C in the dark.

Applications/Dilutions

Application Notes

Plasmacytoid Dendritic Cell (pDC) Kit is validated by Flow Cytometry for identifying pDC from human whole blood, freshly isolated peripheral blood mononuclear cells (PBMC) and frozen PBMC.

Kit Components

Components

CD123 AF647 (human CD123 Alexa Fluor 647 conjugates) antibody 5 ul/test-125 ul hLMAX: Human Lineage Marker Antibody Mix Fixation buffer (1X) 60 ml Mouse IgG1 Isotype control Alexa Fluor 647 conjugate antibody 5 ul/test-125 ul Staining buffer (1X) 1 x 60 ml (Human CD3- CD14- CD16-CD19-CD20- CD56- HLA-DR antibodies)* 10 ul/test-300 ul

Alternate Names for Plasmacytoid Dendritic Cell Assay Kit

• Human Plasmacytoid Dendritic Cell (pDC)
• Human Plasmacytoid Dendritic Cell
• Plasmacytoid Dendritic Cell (pDC)

Background

Plasmacytoid Dendritic Cells (pDC) are a subset of peripheral blood Dendritic Cells which play an important role in both innate and adaptive immune responses. pDC recognize viral components via Toll-like Receptors (TLR) including TLR7 and TLR9, and then rapidly produce Interferon-alpha (IFNalpha) as a primary anti-viral immune response. pDC also participate in antigen presentation, an important functional role which drives the adaptive immune response. Intense investigation has led to pDC identifi cation with a unique set of markers by fl ow cytometry. pDC are usually present in relatively low frequency in peripheral blood. Direct identifi cation of subsets of peripheral blood DCs has an advantage in analyzing changes in number or function of DC subsets during chronic viral infections or diseased patients, as examples.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.