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Intracellular Receptor Staining Flow Cytometry Kit Summary

Kit Type Flow Cytometry Kit

Packaging, Storage & Formulations

Storage Storage is content dependent.

Applications/Dilutions

Application Notes Use in Flow Intracellular reported in scientific literature (PMID 24804954)
Publications
Read Publications using NBP2-29450 in the following applications:
  • FLOW 1 publication
  • Flow-IC 3 publications
  • ICC/IF 1 publication
  • WB 1 publication

Kit Components

Components
  1. Fixation buffer1X 1 X 60 ml
  2. Brefeldin A 1000X 1 X 100 ul
  3. Permeabilization buffer 10X 2 X 60 ml
  4. Staining buffer 1X 2 X 60 ml

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24804954)

Alternate Names for Intracellular Receptor Staining Flow Cytometry Kit

  • Permeabilization buffer

Background

Fixation buffer is used to fix the cells/cellular proteins prior to permeabilization. Permeabilization buffer permeabilizes the cell membrane, allowing the detecting antibodies access to intracellular proteins. It is supplied as a 10X solution and can be diluted with deionized water to its final 1X working concentration. Brefeldin A is an inhibitor of intracellular protein transport. It blocks translocation of proteins from the endoplasmic reticulum to the Golgi apparatus and can enhance detection of some intracellular proteins. Researchers are encouraged to determine whether or not Brefeldin A is needed to assay for the protein of interest. Brefeldin A is light-sensitive, store at 4oC in the dark. It should be diluted in culture media (1:1000) just prior to use. Add to cell culture and incubate for 4hr before harvesting cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Intracellular Receptor Staining Kit (NBP2-29450)(5)

We have publications tested in 2 confirmed species: Human, Canine.We have publications tested in 4 applications: FLOW, Flow-IC, ICC/IF, WB. Submit a Publication Filter By Application FLOW (1) Flow-IC (3) ICC/IF (1) WB (1) All Applications Filter By Species Human (2) Canine (1) All Species Showing Publications 1 - 5 of 5.
Publications using NBP2-29450 Applications Species
Valencia Pacheco Guillermo J, Pinzon Herrera Francisc, Cruz Lopez Juan J et al. Valencia Pacheco Guillermo J, Pinzon Herrera Francisc, Cruz Lopez Juan J et al. Expression and activation of intracellular receptors TLR7, TLR8 and TLR9 in peripheral blood monocytes from HIV-infected patients. Colomb Med (Cali). 2013 Apr [PMID: 24892454] (FLOW) Colomb Med (Cali). 2013 Apr [PMID:24892454] (FLOW) FLOW
Pradhan N, Pratheek BM, Garai A et al. Pradhan N, Pratheek BM, Garai A et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells. Cell Biol. Int. 2014 May 07 [PMID: 24804954] (Flow-IC, Human)Details:Jurkat, Figs 8,9. Cell Biol. Int. 2014 May 07 [PMID:24804954] (Flow-IC, Human) Flow-IC Human
Sathe A, Reddy KV. Sathe A, Reddy KV. TLR9 and RIG-I signaling in human endocervical epithelial cells modulates inflammatory responses of macrophages and dendritic cells in vitro. PLoS ONE. 2014 Jan 10 [PMID: 24409285] (WB, Flow-IC, ICC/IF, Human)Details:End1/E6E7 endocervical epithelial cells: Flow (Intracellular), Fig 1C; IF (confocal microscopy), Fig 1D; WB, Figs S1A, S1C. Both CpG-ODN and Poly(I:C) stimulation upregulated TLR9 expression. TLR9 was detected at ~100 kDa in WB. Mouse monoclonal against T PLoS ONE. 2014 Jan 10 [PMID:24409285] (WB, Flow-IC, ICC/IF, Human) WB, Flow-IC, ICC/IF Human
Chamberlain ND, Kim SJ, Vila OM et al. Chamberlain ND, Kim SJ, Vila OM et al. Ligation of TLR7 by rheumatoid arthritis synovial fluid single strand RNA induces transcription of TNF-alpha in monocytes. Ann Rheum Dis. 2013 Mar [PMID: 22730373] Ann Rheum Dis. 2013 Mar [PMID:22730373]
Burgener IA, Jungi TW. Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008 Jul 15 [PMID: 18439687] (Flow-IC, Canine)Details:Canine (dog) PBMC subpopulations: 1. 10083K [IC-Flow (Intracellular Staining Flow Assay) Kit. Vet Immunol Immunopathol. 2008 Jul 15 [PMID:18439687] (Flow-IC, Canine) Flow-IC Canine

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Product General Protocols

View specific protocols for Intracellular Receptor Staining Kit (NBP2-29450):
  • MSDS (NBP2-29450)
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
  • Flow Cytometry
  • View all Protocols, Troubleshooting, Illustrated assays and Webinars.

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