Intracellular Receptor Staining Flow Cytometry Kit Summary
Kit Type |
Flow Cytometry Kit |
Packaging, Storage & Formulations
Storage |
Storage is content dependent. |
Applications/Dilutions
Application Notes |
Use in Flow Intracellular reported in scientific literature (PMID 24804954) |
Publications |
Read Publications using NBP2-29450 in the following applications: |
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Kit Components
Components
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- Fixation buffer1X 1 X 60 ml
- Brefeldin A 1000X 1 X 100 ul
- Permeabilization buffer 10X 2 X 60 ml
- Staining buffer 1X 2 X 60 ml
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Reactivity Notes
Human reactivity reported in scientific literature (PMID: 24804954)
Alternate Names for Intracellular Receptor Staining Flow Cytometry Kit
Background
Fixation buffer is used to fix the cells/cellular proteins prior to permeabilization.
Permeabilization buffer permeabilizes the cell membrane, allowing
the detecting antibodies access to intracellular proteins. It is supplied
as a 10X solution and can be diluted with deionized water to its final
1X working concentration.
Brefeldin A is an inhibitor of intracellular protein transport. It blocks
translocation of proteins from the endoplasmic reticulum to the Golgi
apparatus and can enhance detection of some intracellular proteins.
Researchers are encouraged to determine whether or not Brefeldin A
is needed to assay for the protein of interest. Brefeldin A is light-sensitive,
store at 4oC in the dark. It should be diluted in culture media (1:1000) just prior to use. Add to cell culture and incubate for 4hr
before harvesting cells.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
Publications for Intracellular Receptor Staining Kit (NBP2-29450)(5)
We have publications tested in 2 confirmed species: Human, Canine.We have publications tested in 4 applications: FLOW, Flow-IC, ICC/IF, WB.
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FLOW (1)
Flow-IC (3)
ICC/IF (1)
WB (1)
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Filter By Species
Human (2)
Canine (1)
All Species
Showing Publications 1 -
5 of 5.
Publications using NBP2-29450 |
Applications |
Species |
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Valencia Pacheco Guillermo J, Pinzon Herrera Francisc, Cruz Lopez Juan J et al. Valencia Pacheco Guillermo J, Pinzon Herrera Francisc, Cruz Lopez Juan J et al. Expression and activation of intracellular receptors TLR7, TLR8 and TLR9 in peripheral blood monocytes from HIV-infected patients. Colomb Med (Cali). 2013 Apr [PMID: 24892454] (FLOW) Colomb Med (Cali). 2013 Apr [PMID:24892454] (FLOW) |
FLOW |
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Pradhan N, Pratheek BM, Garai A et al. Pradhan N, Pratheek BM, Garai A et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells. Cell Biol. Int. 2014 May 07 [PMID: 24804954] (Flow-IC, Human)Details:Jurkat, Figs 8,9. Cell Biol. Int. 2014 May 07 [PMID:24804954] (Flow-IC, Human) |
Flow-IC |
Human |
Sathe A, Reddy KV. Sathe A, Reddy KV. TLR9 and RIG-I signaling in human endocervical epithelial cells modulates inflammatory responses of macrophages and dendritic cells in vitro. PLoS ONE. 2014 Jan 10 [PMID: 24409285] (WB, Flow-IC, ICC/IF, Human)Details:End1/E6E7 endocervical epithelial cells: Flow (Intracellular), Fig 1C; IF (confocal microscopy), Fig 1D; WB, Figs S1A, S1C. Both CpG-ODN and Poly(I:C) stimulation upregulated TLR9 expression. TLR9 was detected at ~100 kDa in WB. Mouse monoclonal against T PLoS ONE. 2014 Jan 10 [PMID:24409285] (WB, Flow-IC, ICC/IF, Human) |
WB, Flow-IC, ICC/IF |
Human |
Chamberlain ND, Kim SJ, Vila OM et al. Chamberlain ND, Kim SJ, Vila OM et al. Ligation of TLR7 by rheumatoid arthritis synovial fluid single strand RNA induces transcription of TNF-alpha in monocytes. Ann Rheum Dis. 2013 Mar [PMID: 22730373] Ann Rheum Dis. 2013 Mar [PMID:22730373] |
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Burgener IA, Jungi TW. Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008 Jul 15 [PMID: 18439687] (Flow-IC, Canine)Details:Canine (dog) PBMC subpopulations: 1. 10083K [IC-Flow (Intracellular Staining Flow Assay) Kit. Vet Immunol Immunopathol. 2008 Jul 15 [PMID:18439687] (Flow-IC, Canine) |
Flow-IC |
Canine |
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Product General Protocols
View specific protocols for Intracellular Receptor Staining Kit (NBP2-29450):
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- Flow Cytometry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars.
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