7-Aminoactinomycin D Solution Summary
| Immunogen |
Appearance (Form) Powder Appearance (Color) Red to Dark Purple Solubility (Solvent) Chloroform Solubility (Conc) 0.95 - 1.05 mg/ml Solubility (Turbidity) Clear Solubility (Color) Red Purity (HPLC) > 95.50 % |
| Specificity |
7-Aminoactinomycin D Solution Molecular Weight 1270.43 |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
| Buffer |
PBS and 1.0% FBS |
| Preservative |
0.09% Sodium Azide |
Applications/Dilutions
| Application Notes |
Fluorescent DNA stain7- Amino-Actinomycin D (7-AAD) can be used as a viability probe for methods of nonviable cell exclusion based on light scatter and uptake of the reagent as detected in FL3. 7-AAD is excluded by viable cells but can penetrate cell membranes of dying or dead cells, in which it intercalates into double stranded nucleic acids. It can be used in place of Propidium Iodide (PI) for omission of nonviable cells in flow cytometric assay. The advantage of 7-AAD over PI is that detection is in the far red range of the spectrum (650 nm long-pass filter) and is read in the FL3, compared to PI detection in FL2. This allows this solution to be used in combination with PE (phycoerythrin), FITC (fluorescein isothiocyanate), and APC (allophycocyanin) conjugated antibodies, as well as any other conjugated antibody that does not emit into FL3. |
| Publications |
| Read Publications using NBP2-29446 in the following applications: |
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Alternate Names for 7-Aminoactinomycin D Solution
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
Publications for 7-Aminoactinomycin D Solution (NBP2-29446)(2)
We have publications tested in 2 confirmed species: Human, Mouse.We have publications tested in 1 application: Flow-IC.
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Flow-IC (2)
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Showing Publications 1 -
2 of 2.
| Publications using NBP2-29446 |
Applications |
Species |
|---|
| Kanehira M, Kikuchi T, Santoso A et al. Kanehira M, Kikuchi T, Santoso A et al. Human marrow stromal cells downsize the stem cell fraction of lung cancers by fibroblast growth factor 10. Mol. Cell. Biol. 2014 Aug 01 [PMID: 24865969] (Flow-IC, Human)Details:Figs 1, 7: H1299, A549, H1975, MCF7, & HeLa cells. Mol. Cell. Biol. 2014 Aug 01 [PMID:24865969] (Flow-IC, Human) |
Flow-IC |
Human |
| Sakurai Y, Hatakeyama H, Sato Y et al. Sakurai Y, Hatakeyama H, Sato Y et al. RNAi-mediated gene knockdown and anti-angiogenic therapy of RCCs using a cyclic RGD-modified liposomal-siRNA system. J Control Release. 2014 Jan 10 [PMID: 24120854] (Flow-IC, Mouse) J Control Release. 2014 Jan 10 [PMID:24120854] (Flow-IC, Mouse) |
Flow-IC |
Mouse |
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Product General Protocols
View specific protocols for 7-Aminoactinomycin D Solution (NBP2-29446):
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Flow Cytometry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars.
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Additional 7-Aminoactinomycin D Solution Products
7-Aminoactinomycin D Solution NBP2-29446
- 7-Aminoactinomycin D Solution Support Product
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