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Annexin V Apoptosis Kit [FITC] Summary

Kit Type Apoptosis Kit
Gene ANXA5

Packaging, Storage & Formulations

Storage Store at 4C. Do not freeze.
Conjugate FITC
Format FITC (A=495, E=519)

Applications/Dilutions

Application Notes Use in Flow cell surface reported in scientific literature (PMID 23955790) Use the Control Cells provided to set up compensation and quadrants. The Control Cells are positive for both Annexin V-FITC and PI. The basal level of apoptosis and necrosis varies considerably within a give cell population. Even in the absence of induced apoptosis, most cell populations will contain a minor percentage of cells that are positive for apoptosis ((Annexin V-FITC positive, PI negative) and dead, necrotic, or in the late stages of apoptosis (Annexin V-FITC positive, PI positive). Thus an untreated cell population is used to define the basal level of apoptotic and dead cells. Determine the percentage of cells that have been induced to undergo apoptosis by subtracting the percentage of apoptotic cells in the untreated from the treated population. Since cell death is the eventual outcome of cells undergoing apoptosis, cells in the late stages of apoptosis will have a damaged membrane and stain positive for PI as well as for Annexin V-FITC. Consequently cells which have undergone necrosis are not distinguishable from those which have undergone apoptosis. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25947082). Use in FLOW cytometry reported in scientific literature ( PMID 27448441)
Publications
Read Publications using NBP2-29373 in the following applications:
  • FLOW 8 publications
  • Flow - CS 1 publication
  • Flow-CS 5 publications
  • ICC/IF 2 publications

Kit Components

Components
  1. Control Cells: 1 x 10^6 cells/ml
  2. Annexin V-FITC
  3. Propidium Iodide (PI)
  4. 10X Binding Buffer
  5. 10X PBS

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23955790) Mouse reactivity reported in scientific literature (PMID: 22566271). Rat reactivity reported in scientific literature (PMID: 25947082)

Notes

ADDITIONAL ITEMS REQUIRED (NOT INCLUDED IN THE KIT) Distilled H2O Flow Cytometer Experimental Cells Centrifuge Positive Control Cell Line (recommended, not required)

Alternate Names for Annexin V Apoptosis Kit [FITC]

  • Anchorin CII
  • annexin A5
  • Annexin V
  • annexin-5
  • ANX5
  • ANXA5
  • ANXV
  • Calphobindin I
  • Endonexin II
  • ENX2CBP-I
  • Lipocortin V
  • PAP-I
  • Placental anticoagulant protein 4
  • Placental anticoagulant protein I
  • PP4
  • Thromboplastin inhibitor
  • VAC-alpha
  • Vascular anticoagulant-alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Annexin V Kit (NBP2-29373)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.We have publications tested in 4 applications: FLOW, Flow - CS, Flow-CS, ICC/IF. Submit a Publication Filter By Application FLOW (8) Flow - CS (1) Flow-CS (5) ICC/IF (2) All Applications Filter By Species Human (13) Mouse (2) Rat (2) All Species Showing Publications 1 - 10 of 19. Show All 19 Publications. Collapse Publications.
Publications using NBP2-29373 Applications Species
Esposito CL, Nuzzo S, Kumar SA et al. Esposito CL, Nuzzo S, Kumar SA et al. A combined microRNA-based targeted therapeutic approach to eradicate glioblastoma stem-like cells J Control Release 2016 Jul 21 [PMID: 27448441] (FLOW, Human) J Control Release 2016 Jul 21 [PMID:27448441] (FLOW, Human) FLOW Human
Chen Y, Huang H, Yao C et al. Chen Y, Huang H, Yao C et al. Antitumor activity of combined endostatin and thymidine kinase gene therapy in C6 glioma models Cancer Med 2016 Jul 01 [PMID: 27366865] Cancer Med 2016 Jul 01 [PMID:27366865]
Hartung F, Pardo LA. Hartung F, Pardo LA. Guiding TRAIL to cancer cells through Kv10.1 potassium channel overcomes resistance to doxorubicin Eur. Biophys. J. 2016 Jun 27 [PMID: 27350552] (ICC/IF, Human) Eur. Biophys. J. 2016 Jun 27 [PMID:27350552] (ICC/IF, Human) ICC/IF Human
Huang CL, Chao CC, Lee YC et al. Huang CL, Chao CC, Lee YC et al. Paraquat Induces Cell Death Through Impairing Mitochondrial Membrane Permeability. Mol. Neurobiol. 2015 May 07 [PMID: 25947082] (ICC/IF, FLOW, Rat)Details:Annexin V-FITC-conjugated apoptosis kit was used for analyzing apoptotic cells in experiments involving PC12 cells. The cells with or without 24 hours of Paraquat/PQ treatments (+ dibucaine or cyclosporin A treatments) were subjected to Annexin V-FITC-staining followed by microscopgy imaging or flow cytometric analysis (Fig 1D). Mol. Neurobiol. 2015 May 07 [PMID:25947082] (ICC/IF, FLOW, Rat) ICC/IF, FLOW Rat
Xue Y, Wu L, Liu Y et al. Xue Y, Wu L, Liu Y et al. ENTPD5 Induces Apoptosis in Lung Cancer Cells via Regulating Caspase 3 Expression PLoS ONE 2015 Mar 21 [PMID: 25794010] (FLOW, Human) PLoS ONE 2015 Mar 21 [PMID:25794010] (FLOW, Human) FLOW Human
Song W, Soo Lee S, Savini M et al. Song W, Soo Lee S, Savini M et al. Ceria nanoparticles stabilized by organic surface coatings activate the lysosome-autophagy system and enhance autophagic clearance. ACS Nano. 2014 Oct 28 [PMID: 25315655] (FLOW, Human)Details:Annexin V - FITC Apoptosis Kit (Imgenex's CytoGLO Annexin V-FITC Apoptosis Detection Kit 10085K) used for detecting - Annexin V binding affinity change (%) in HeLa/TFEB cells treated or not with CeO2 nanoparticles or taxol for 24 h (Figure 3A). ACS Nano. 2014 Oct 28 [PMID:25315655] (FLOW, Human) FLOW Human
Song W, Wang F, Lotfi P et al. Song W, Wang F, Lotfi P et al. 2-Hydroxypropyl-B-Cyclodextrin Promotes TFEB-mediated Activation of Autophagy: Implications for Therapy. J. Biol. Chem. 2014 Feb 20 [PMID: 24558044] (Flow-CS, Human) J. Biol. Chem. 2014 Feb 20 [PMID:24558044] (Flow-CS, Human) Flow-CS Human
Kumar A, Kant S, Singh SM. Kumar A, Kant S, Singh SM. Targeting monocarboxylate transporter by alpha-cyano-4-hydroxycinnamate modulates apoptosis and cisplatin resistance of Colo205 cells: implication of altered cell survival regulation. Apoptosis 2013 Dec [PMID: 23955790] (Flow-CS, Human) Apoptosis 2013 Dec [PMID:23955790] (Flow-CS, Human) Flow-CS Human
Li FJ, Schreeder DM, Li R et al. Li FJ, Schreeder DM, Li R et al. FCRL3 promotes TLR9-induced B-cell activation and suppresses plasma cell differentiation. Eur J Immunol 2013 Nov [PMID: 23857366] (FLOW, Human)Details:Purified human blood B cells, Fig 2C. Eur J Immunol 2013 Nov [PMID:23857366] (FLOW, Human) FLOW Human
Wang F, Segatori L. Wang F, Segatori L. Remodeling the proteostasis network to rescue glucocerebrosidase variants by inhibiting ER-associated degradation and enhancing ER folding. PLoS One. 2013 [PMID: 23620750] (FLOW, Human)Details:Annexin V - FITC Apoptosis Kit (Imgenex's CytoGLO Annexin V-FITC Apoptosis Detection Kit ) used for detecting - Annexin V binding in lacidipine and EerI treated GD cells, Gaucher?s disease /GD patient-derived fibroblasts homozygous for the L444P (1448T>C) mutation (GM10915). FLOW data is shown in Figure 3A. PLoS One. 2013 [PMID:23620750] (FLOW, Human) FLOW Human
Show All 19 Publications. Collapse Publications.

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Product General Protocols

View specific protocols for Annexin V Kit (NBP2-29373):
  • MSDS for Non Hazardous Product (NBP2-29373)
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • View all Protocols, Troubleshooting, Illustrated assays and Webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Annexin V Kit (NBP2-29373) (0)

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Bioinformatics Tool for Annexin V Kit (NBP2-29373)

Discover related pathways, diseases and genes to Annexin V Kit (NBP2-29373). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.

Diseases for Annexin V Kit (NBP2-29373)

Discover more about diseases related to Annexin V Kit (NBP2-29373).
  • Inflammation
  • Thrombosis
  • Malignant Neoplasms
  • Neoplasms
  • Atherosclerosis
  • Antiphospholipid Syndrome
  • Carcinoma
  • Acute Promyelocytic Leukemia
  • Lupus Erythematosus, Systemic
  • Pregnancy Complications
  • Thrombophilia
  • Cardiovascular Diseases
  • Neoplasm Metastasis
  • Polyendocrinopathies, Autoimmune
  • Autoimmune Reaction
 

Pathways for Annexin V Kit (NBP2-29373)

View related products by pathway.
  • Coagulation
  • Cell Death
  • Pathogenesis
  • Localization
  • Immune Response
  • Phagocytosis
  • Cell Differentiation
  • Conjugation
  • Exocytosis
  • Hemostasis
  • Blood Coagulation
  • Cell Proliferation
  • Endocytosis
  • Methylation
  • Regeneration
  • Secretion
  • Cell Adhesion
  • Transport
  • Transposition
  • Programmed Cell Death
  •  

PTMs for Annexin V Kit (NBP2-29373)

Learn more about PTMs related to Annexin V Kit (NBP2-29373).
  • Phosphorylation
  • Methylation
  • Carboxylation
  • Reduction
  • Dephosphorylation
 

Research Areas for Annexin V Kit (NBP2-29373)

Find related products by research area.
  • Apoptosis
  • Apoptosis Detections

Blogs on Annexin V

There are no specific blogs for Annexin V, but you can read our latest blog posts.