Permits and Restrictions |
View Permits |
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Organism | Homo sapiens, human |
Components | ATCC CCL-224 COLO 201ATCC CCL-238 SW1417ATCC CRL-11147 A2058ATCC CRL-2577 RKOATCC CRL-7898 A101DATCC HTB-123 DU4475ATCC HTB-137 Hs 695TATCC HTB-66 RPMI-7951 |
Applications |
This validated panel is composed of 8 select human tumor cell lines carrying BRAF mutations, and is useful for BRAF pathway research and for anti-cancer drug discovery. |
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Comments |
BRAF gene is a proto-oncogene encoding the BRAF protein, a serine/threonine kinase of the RAF family that acts downstream of RAS and upstream of MEK in the MAPK/ERK signaling pathway. BRAF mediates cell division, proliferation, and differentiation in response to a host of stimuli. Mutations in BRAF lead to excessive cellular proliferation and enhanced survival, and often underlie birth defects, and cancers of the thyroid and skin. The BRAF gene is most frequently mutated at codon 600. BRAF V600E mutation has been found in more than 60% of melanomas, as well as 7-8% of other cancers. The BRAF Genetic Alteration Cell Panel (ATCC TCP-1032) is composed of eight selected human tumor cell lines from various common cancer types that carry BRAF hotspot mutations in codon 600. The BRAF mutation status of each cell line has been sequenced and validated by ATCC. This panel is useful for BRAF pathway research and BRAF inhibitors anti-cancer drug discovery. |
Product Format | 8 cryopreserved cell lines |
Storage Conditions | liquid nitrogen vapor phase (less than -130°C) only |
Permits |
These permits may be required for shipping this product to Australia:
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Basic Documentation | Product Sheet Certificate of Analysis SDS |
Other Documentation | Recommended Culture Conditions Genetic Alteration Panels - Brochure |