Permits and Restrictions |
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Organism | Homo sapiens, human |
Tissue | Foreskin |
Cell Type | Fibroblast |
Morphology | Spindle-shaped; cells are bipolar and refractile. |
Growth Properties | Adherent; mitotically arrested. |
Biosafety Level |
1 [These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens. ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC) (Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223. Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.] Human Material PrecautionAll tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease | Normal |
Age | Neonatal |
Gender | Male |
Applications | The cells can be used as a feeder layer to support the growth of embryonic stem (ES) cells and for the maintenance of ES cells in the undifferentiated state. Suitable for use with other cell types that require a feeder layer for optimal attachment and proliferation. |
Product Format | frozen 1 mL |
Storage Conditions | -130°C or below |
Comments | Primary neonatal fibroblasts have been treated with mitomycin C at passage #1 and will not replicate. Once the feeder cells have attached, the culture medium should be changed to accommodate the cells to be supported. |
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Complete Growth Medium |
Table 1. If using the Fibroblast Growth Kit–Serum-Free (ATCC PCS-201-040), add the indicated volume for each component in the order shown.
Table 2. If using the Fibroblast Growth Kit–Low Serum (ATCC PCS-201-041), add the indicated volume for each of the following components:
Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of either of the optional components (GA solution or PSA solution) to be added to the complete growth media is summarized in Table 3.
Table 3. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)
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Volume | 1 mL |
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Cells per Vial | One vial contains a minimum of 3 x 106 viable cells. |
Sterility Tests | Bacteria and Yeasts: Negative. Mycoplasma: Negative |
Viral Testing | HIV: Negative Hepatitis B: Negative Hepatitis C: Negative |
Viability | ≥70% when thawed from cryopreservation |
Population Doubling Capacity | No growth/division over 4 weeks |
C of A | Certificate of Analysis |
Permits |
These permits may be required for shipping this product to Australia:
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Basic Documentation | Product Sheet Certificate of Analysis SDS |