Permits and Restrictions |
View Permits |
---|---|
Organism | Homo sapiens, human |
Tissue | pulmonary artery endothelium |
Cell Type | endothelial cells |
Morphology | Cobblestone appearance with large dark nuclei; during proliferation, cells are small and evenly sized, display a high mitotic index and show no presence of smooth muscle cells. |
Growth Properties | Adherent |
Biosafety Level |
1 [These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens. ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC) (Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223. Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.] Human Material PrecautionAll tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome. Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Disease | normal |
Age | Lot-specific |
Gender | Lot-specific |
Ethnicity | Lot-specific |
Applications | Angiogenesis, arteriolosclerosis, hypertension, oncology, drug screening, inflammation, vasodilator function. |
Product Format | frozen 1 mL |
Storage Conditions | -130°C or below |
Comments | The cells are cryopreserved in the second passage to ensure the highest purity, coupled with high viability and plating efficiency. A complete solution to propagate pulmonary artery endothelial cells in low serum conditions with or without the addition of human recombinant VEGF. Use of the Endothelial Cell Growth Kit-VEGF will support a faster rate of proliferation because of the presence of several purified recombinant human (rh) growth factors (rh VEGF, rh EGF, rh FGF basic and rh IGF-1) combined with heparin and hydrocortisone. Use of the Endothelial Cell Growth Kit-BBE, which contains Bovine Brain Extract (BBE), is recommended if a more "authentic," less defined culture medium is desired. |
---|
Complete Growth Medium |
Table 1. If using the Endothelial Cell Growth Kit-BBE (ATCC® PCS-100-040), add the indicated volume for each component:
Table 2. If using the Endothelial Cell Growth Kit-VEGF (ATCC® PCS-100-041), add the indicated volume for each component:
Antimicrobials and phenol red are not required for proliferation but may be added if desired. The recommended volume of either of the optional components (GA solution or PSA solution) to be added to the complete growth media is summarized in Table 3.
Table 3. Addition of Antimicrobials/Antimycotics and Phenol Red (Optional)
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Subculturing |
|
Volume | 1 mL |
---|---|
Cells per Vial | One vial contains a minimum of 5 x 105 viable cells. |
Sterility Tests | Bacteria and Yeast: NegativeMycoplasma: Negative |
Viral Testing | Hepatitis B: Negative Hepatitis C: Negative HIV-1: Negative HIV-2: Negative |
Viability | ≥ 70% when thawed from cryopreservation. |
Passage Number | These cells are cryopreserved at the second passage |
Population Doubling Time | ≥ 15 in complete growth medium |
C of A | Certificate of Analysis |
Permits |
These permits may be required for shipping this product to Australia:
|
---|---|
Basic Documentation | Product Sheet Certificate of Analysis SDS |