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Infectious Materials

Infectious diseases are caused by biological agents, and produce clinically evident illness in humans. They can be transmitted through a variety of routes, including bodily fluids, animal or insect vectors, contaminated food and water or from human-to- human contact. By partnering with ATCC and ACDBio, In Vitro Technologies offers model pathogens, nucleic acid controls and in situ hybridization- based methods for detecting infection in cells and tissues.

The extraction, preparation, and quality control of agents for infectious disease research can often require extensive amounts of time, labour, and expense. This is particularly true when handling cultures that require advanced biological safety procedures, fastidious culturing conditions, or extensive incubation periods.

Through our partnership with the American Tissue Culture Collection (ATCC), In Vitro can offer researchers in Australia and New Zealand both original viral isolates as well as both genuine and synthetic nucleic acids to accelerate research into infectious diseases.

  • ATCC Genuine Cultures are backed by meticulous laboratory procedures and represent ATCC's high standards of quality for microbial strains, including full characterization of each strain to establish identity, utilization of a seed stock system to minimize subculturing, and careful preservation for culture maintenance.
  • ATCC Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays.
  • ATCC Synthetic Nucleic acids are designed and developed by the experts in microbial genomics at ATCC to include key target regions from difficult-to culture or unculturable microorganisms. Each preparation is supported by stringent quality control analyses to ensure product identity, stability, quantity, and functionality. These molecular standards are appropriate for use as control material in the development and validation of molecular-based applications.

ATCC sti2

ATCC respiratory2

ATCC vectorborne 2

ATCC enteric2

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Detecting Infection

In-situ hybridisation of RNA (ISH) is a powerful technique that compliments PCR data with single-cell expression level and localisation. To date however, ISH has lacked sensitivity and robustness. In 2011 scientists from ACDbio revolutionized the RNA in situ hybridization method and introduced RNAscope ISH for detection of target RNA within intact cells. The assay represents a major advance in RNA ISH approaches with its proprietary probe design that simultaneously amplifies target-specific signals and suppresses background noise from non-specific hybridization. Known for its sensitivity and specificity, RNAscope ISH is now a proven technology that has been featured in over 500 publications across a multitude of research areas.

hivDespite the remarkable success of antiretroviral therapy (ART), the persistence of HIV reservoirs is a major barrier to overcome in finding a lasting cure. In order to completely eradicate HIV and advance therapeutic interventions, it is important to elucidate the biological nature of viral latency as well as productively infected cells in tissues including the gastrointestinal tract and those of the lymphatic system. RNAscope, a highly specific and sensitive in-situ hybridization technology, employs a powerful technique to study HIV and SIV in cellular and tissue environments.

zikaZika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia, Africa and recently the Americas. Isolated in 1947 from a rhesus monkey in the Zika forest of Uganda, it is believed to spread to humans via two types of mosquitos--Aedes aegypti and Aedes albopictus. Human infection leads to a variable self-limited illness with mild to severe symptoms well described in current literature and media. As a result of the current Zika virus outbreak, researchers are accelerating research into the biology of the virus and the pathology of human infection with the goals of understanding the causes of the clinical syndrome, developing reliable detection methods, and finding effective treatments

infectiousDirect detection of viral RNA in human or other animal cells by RNA in situ hybridization (ISH) is a powerful tool to establish the etiology and pathogenesis of viral diseases. Nucleic acid-based molecular detection methods have revolutionized viral detection, offering several essential advantages such as sensitivity, specificity and speed. Beyond those stated advantages, RNAscope ISH uniquely offers molecular detection coupled with morphological context enabling visualization of the virus in different infected tissues and cell types.

hpvEvidence for transcriptionally active HPV oncogenes E6/E7 is regarded as the gold standard for presence of clinically relevant high-risk human papillomavirus (HPV), but detection of E6/E7 mRNA can be challenging using conventional techniques. As a causal agent in head and neck squamous cell carcinoma (HNSCC), it is critical that the detection method enable pathologist review of tissue morphology and be of the highest specificity and sensitivity for accurate assessment of within the tissue microenvironment of FFPE specimens. RNAscope HPV Biomarker Detection Reagents and its proprietary “double Z” oligonucleotide probes specific for each subtype E6/E7 mRNA enable high specificity detection of viral transcripts in routine FFPE tumor biopsies.

inflammationUnderstanding molecular mechanisms is critical for identifying pathogenesis, evaluating disease progression and developing new therapeutic strategies for autoimmunity, infectious diseases, and cancer. With RNAscope ISH assay, you can detect specifically expressed immune markers in lymphoid and non-lymphoid tissues while maintaining the morphological context.

Play and Publish Cell Imaging

With the iRiS Digital Cell Imaging System, you can obtain high resolution, publication-quality images without the complex steps necessary with a conventional fluorescence microscope. This system combines high performance optics, automation, and a user-friendly workflow to create a seamless user experience. High quality images can be captured consistently regardless of a user’s level of experience. With the iRiS Digital Imaging System, novices, experts, and everyone in between can easily obtain high quality crisp images in seconds.

This intelligent system, complete with time lapse and z-stack imaging capability along with on-board intuitive analysis software enables you to bring your digital imaging to a new level.